PARENT SESSION
Poster Session # 21: Biogeography, Biodiversity, Populations, and Genetics.
Thursday, August 7 Presentation from 5:00 PM to 6:30 PM. SITCC Exhibit Hall B.
Microsatellite DNA variation of Peromyscus maniculatus populations in New Mexico.
Rubio, Maria*,1, Dragoo, Jerry1, Yates, Terry1, 1 University of New Mexico, Albuquerque, New Mexico, USA
ABSTRACT- The deer mouse (Peromyscus maniculatus) is a common and widely distributed rodent known to transmit the hantavirus, Sin Nombre virus (SNV). Genetic studies have revealed strong subdivisions among rodent populations, even at relatively fine scales. We hypothesize that genetic variation among neighboring populations may be an effect of extinction and then recolonization from a surrounding area. Analysis of the resident population through time may shed some light on the origin of dispersers. Microsatellite data when analyzed in the context of longitudinal studies will allow us to investigate the basic genetic profile for the host population with an estimation of how it changes over time. Ultimately, we plan to use these data to test various models associated with the epidemiology of SNV. We have established parameters required to amplify 12 Peromyscus microsatellite loci using DNA extracted from ear punches from P. maniculatus located on 2 study sites in northwestern New Mexico. The loci we have examined were obtained from Chirhart et al. (2000 - Mol. Ecol., 9:1669). The 12 loci are as follows: Pml01 (125-195 bp), Pml02 (215-251 bp), Pml03 (224-273 bp), Pml04 (196-233 bp), Pml05 (191-225), Pml06 (129-197 bp), Pml07 (193-264 bp), Pml08 (204-256 bp), Pml09 (193-264 bp), Pml10 (162-198 bp), Pml11 (213-283 bp), Pml12 (139-187 bp). As of 1 March 2003 we have analyzed approximately 50 of 98 animals for which we have DNA extracted. All l2 loci were polymorphic and average 20 alleles per locus. Expected heterozygosity was 0.92 whereas observed heterozygosity was 0.76. We will present population genetic data on approximately 100 animals that have been marked and recaptured monthly from 2000 to 2002.
Key words: microsatellite DNA, Peromyscus maniculatus, population genetics