PARENT SESSION
Poster Session 30: Soil Ecology.
Wednesday, August 4 Presentations from 5:00 PM to 6:30 PM, Exhibit Hall A 1.
Influence of extraction protocol and extent land-use on soil lipid profiles.
Boser, Christina*,1, Fujinuma, Ryosuke1, Read, Harry1, Balser, Teri1, 1 University of Wisconsin - Madison, Madison, Wisconsin, USA
ABSTRACT- We tested two modifications of the Bligh and Dyer method on six varieties of soil types under different land-use to improve the quality and quantity of results for lipid analysis such as phospho-lipid fatty acids (PLFA). We examined this with modification of the volume of chloroform and addition of the rinsing process. The first experiment suggested that the optimal volume of chloroform for extraction was dependent on the quantity of soil organic matter in the sample. It confirmed that the lipid analysis may be required to differentiate the extraction process by soil types. The second experiment suggested that three repetitions of the chloroform extraction would increase lipid yield to up to 90% of extractable lipids. Also the repetitions help to reduce the differentiation of quantities of extracted lipids by soil types. Our result implies the repetition of chloroform extraction is crucial in order to standardize the lipid analysis, which recommended for use in experiments that require data on both soil microbial biomass and microbial community composition profile.
Key words: soil microbial community, soil lipid profile, land-use