PARENT SESSION
Poster Session 20: Invasive Species
Wednesday, August 10, 5:00 PM - 6:30 PM, Exhibit Hall 220 A-E, Level 2, Palais des congrès de Montréal
Asian lineage of the new subspecies Phragmites australis subsp. americanus Saltonstall, P.M. Peterson & Streng., based on RFLP analysis of chloroplast non-coding DNA regions.
Wing, Claudia*,1, Frohnapple, Paul*,1, Chun, Young-Moon1, Tseng, Charles1, Choi, Young1, 1 Department of Biological Sciences, Hammond, IN, USA
ABSTRACT- Distribution and abundance of European haplotype (M) Phragmites australis (Cav.) Trin. Ex has increased significantly in North America in the past decades, and the native North American haplotype has been recognized as a new subspecies Phragmites australis subsp. americanus Saltonstall, P.M. Peterson & Streng. We compared 2 chloroplast non-coding DNA regions of this new subspecies to the Type M and Asian samples of Phragmites australis that were collected in South Korea. Our RFLP analysis with PCR revealed that the North American natives were more similar to the Korean populations than the Type M. The products resulting with using trnLb amplification resulted in a 350-bp band. Analysis with HhaI restriction endonuclease then produced a 282-bp band that distinguished the M haplotype, while the North American and Korean samples remained uncut. We confirmed this discrepancy with rbcL primer pairs. A digestion with Rsa I enzyme produced a 246-bp band that left the M uncut while the North American and Korean cut. These results suggest that the North American haplotype may have a closer evolutionary lineage with Asian Phragmites australis than with the European Type M.
Key words: Phragmites australis, invasive species, RFLP analysis, Phragmites subspecies