PARENT SESSION
Contributed Oral Session 111: Biodiversity: Genetics and Evolution
Thursday, August 11, 8:00 AM - 11:30 AM, Meeting Room 513 E, Level 5, Palais des congrès de Montréal
Targeted characterisation of Trifolium spumosum: ecogeographical and molecular approaches.
Ghamkhar, Kioumars*,1, Snowball, Richard2, Bennett, Sarita3, 1 Centre for Legumes in Mediterranean Agriculture, Crawley, WA, Australia2 Department of Agriculture Western Australia, South Perth, WA, Australia3 CRC for Plant-based Management of Dryland Salinity, Crawley, WA, Australia
ABSTRACT- A core collection is a sub-set encompassing more than 70% of the variability of all accessions held in a collection. Ecogeographical and molecular are significant traits in generating the best core collection. However, molecular techniques including Amplified Fragment Length Polymorphisms (AFLPs), microsatellites and isozymes have rarely been used for this purpose in legumes. The aim of this work is to develop a core collection of Trifolium spumosum as a model for other pasture legume species. Accessions with near complete ecogeographical data were selected from the Australian ex situ collection of Trifolium spumosum. This collection of 317 accessions was stratified into 5 geographical regions. MStrat Software was used to select the preliminary core of 30% of the collection (105 accessions). Among the most diverse accessions in the first subset, 20 were studied using Fluorescent Amplified Fragment Length Polymorphisms (FAFLPs). Each was screened using five AFLP repeats. Eight primer pairs producing the highest number of bands were selected and are being used to screen the preliminary core collection. Mstrat and PAUP* will be used to develop final core collections containing 30% of the preliminary subset. This core collection of 32 accessions will be selected using AFLP and ecogeographical data. The final core collection should cover the highest diversity with lowest repetition within the smallest collection size. This research will also identify deficiencies in genetic diversity resulting in better targeted collecting.
Key words: Core collection, Ecogeography, AFLP, Trifolium spumosum