PARENT SESSION
Poster Session 12: Physiology
Tuesday, August 9, 5:00 PM - 6:30 PM, Exhibit Hall 220 A-E, Level 2, Palais des congrès de Montréal
The effect of NaCl on the growth and shoot production of Musa in vitro.
Macedo, Cristiane1, Barroso, Paulo2, Dantas, Gioconda 3, Alloufa, Magdy4, 1 Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil2 Empresa Brasileira de Pesquisa Agropecuaria - EMBRAPA, Campina - Grande, PB, Brazil3 Universidade Federal do Rio Grande do Norte, Natal, RN4 Universidade Federal do Rio Grande do Norte, Natal, RN
ABSTRACT- The effect of different sodium chloride (NaCl) concentrations, in vitro, on the growth and multiplication shoot derived from the explants of Grand Naine variety was studied.The shoots of bananeira were inoculated in MS media in the absence: T0=0 (controle) and in the presence of three NaCl treatments: T1= 50mM, T2=75mM e T3=100mM and subcultured five times in the respective treatments. The subcultures were carried out every 30 days and the number of shoots multiplicated on each inoculated shoot, length and total number leaves of inoculated shoot and production of fresh weight were observed at 0; 30; 60; 90; 120 e 150 days after salt exposition. The results showed that the presence of NaCl in the media affects the shoot production in vitro. Increase of NaCl concentration in the media is proportional to decrease in the number of shoots produced. Shoot multiplication was reduced about 80% in the treatment T1, while the treatments T2 and T3 achieved until 90% of reduction. On the other hand the wo salines treatments 75 and 100 mM interfered at same form in the growth of shoots, production of fresh weight, length and total number leaves. Under experimental conditions used the best dose among the different sodium chloride considered treatments to later selections was 50 mM account of to had showed intermediary effects.
Key words: Musa sp., salt stress, in vitro multiplication