PARENT SESSION Posters P2A Type I reaction centres. Abstracts (181-218)
(P2A-1) Mutational analysis of the two phylloquinones in Photosystem I. (Abstract 181) Feifei Gu*,1, Martin Byrdin2, Fabrice Rappaport2, Art van der Est3, Fraser MacMillan4, Kevin Redding1, 1 University of Alabama, Tuscaloosa, AL, USA2 Institut de Biologie Physico-Chimique, Paris, France3 Brock University, Ontario, Canada4 J.W. Goethe University, Frankfurt am Main, Germany (P2A-2) Spectroscopic studies of electron transfer reactions in membranes and isolated reaction center from Heliobacterium modesticaldum. (Abstract 182) Hirozo Oh-oka*,1, Masayo Iwaki2, Ryo Miyamoto3, Hiroyuki Mino3, Shigeru Itoh3, 1 Department of Biology, Graduate School of Science, Osaka University, Osaka, Japan2 Department of Biology, University College London, London, UK3 Division of Material Science, Graduate School of Science, Nagoya University, Nagoya, Japan (P2A-3) Temperature dependence of charge recombination in Photosystem I trimers embedded in trehalose glass. (Abstract 183) Rufat Agalarov*,1, Ramakrishnan Balasubramanian1, John Golbeck1, 2, 1 Department of Biochemistry and Molecular Biology, University Park, PA, USA2 Department of Chemistry, Pennsylvania State University, University Park, PA, USA (P2A-4) Iron sulfur cluster assembly in Photosystem I: The role of the transcriptional repressor SufR in regulating the suf regulon in cyanobacterium Synechococcus sp. PCC 7002. (Abstract 184) Ramakrishnan Balasubramanian*,1, Tao Wang1, Gaozhong Shen1, Lee Hoffart1, Carsten Krebs1, 2, Lee McIntosh3, Donald Bryant1, John Golbeck1, 2, 1 Department of Biochemistry and Molecular Biology, University Park, PA, United States2 Department of Chemistry, University Park, PA, United States3 MSU-DOE Plant Research Laboratory, East Lansing, MI, United States (P2A-5) Red absorption changes accompanying electron transfer from A1 to the iron-sulfur clusters in Photosystem I. (Abstract 185) Krzysztof Dobek1, 2, Klaus Brettel*,2, 1 Quantum Electronics Division, Department of Physics, Poznan, Poland2 Service de Bioenergetique, Gif-sur-Yvette Cedex, France (P2A-6) Exploring the rate of forward electron transfer in PSI with a series of anthraquinones in the A1 binding site. (Abstract 186) Sarah Brown*,1, Julia Pushkar2, Irina Karygina2, Dietmar Stehlik2, Art van der Est1, 1 Brock University, St. Catharines, Ontario, Canada2 Institut für Experimentalphysik, Berlin, Germany (P2A-7) EPR study on the arrangement of the terminal [4Fe-4S] clusters in the reaction center of green sulfur bacteria. (Abstract 187) K. Lakshmi*,1, Wade Johnson2, John Golbeck2, Gary Brudvig1, 1 Department of Chemistry, New Haven, CT, USA2 Department of Biochemistry and Molecular Biology, University Park, PA, USA (P2A-8) Antenna ring around trimeric Photosystem I in a photosynthetic prokaryote Prochlorothrix hollandica. (Abstract 188) Ladislav Bumba*,1, 3, Ondrej Prasil2, Frantisek Vacha2, 3, 1 Faculty of Biological Sciences, Ceske Budejovice, Czech republic3 Institute of Plant Molecular Biology, Ceske Budejovice, Czech republic2 Institute of Physical Biology, Nove Hrady, Czech republic (P2A-9) Methionine to asparagine mutations of the Ao chlorophyll ligand in the PsaA and PsaB subunits of Photosystem I in Synechocystis sp. PCC 6803. (Abstract 189) Rachel Cohen*,1, Wu Xu2, Parag Chitnis2, John Golbeck1, 1 1 Department of biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA, 16802, USA2 Department of Biochemistry, Molecular Biology, and Biophysics, Iowa State University, Ames, IA, 50010, USA (P2A-10) Probing the active electron transfer branch in Photosystem I reaction center from Synechocystis sp. PCC 6803 by means of ultrafast spectroscopy. (Abstract 190) Naranbaatar Dashdorj*,1, Wu Xu2, John Golbeck3, Parag Chitnis2, Sergei Savikhin1, 1 Department of Physics, West Lafayette, IN, USA2 Department of Biochemistry, Biophysics, and Molecular Biology, Ames, IA, USA3 Department of Biochemistry and Molecular Biology, University Park, PA, USA (P2A-11) Measurement of electron transfer via both phylloquinones in the Photosystem I reaction centre of Chlamydomonas. (Abstract 191) Peter Heathcote*,1, Stefano Santabarbara1, Ilya Kuprov2, Wendy Fairclough1, Peter Hore2, Saul Purton3, Michael Evans3, 1 School of Biological Sciences, London, UK2 Department of Chemistry, Oxford, UK3 Department of Biology, London, UK (P2A-12) Electron paramagnetic resonance spectra and protein composition of photosynthetic reaction centers from Heliobacterium modesticaldum. (Abstract 192) Mark Heinnickel*,1, Gaozhong Shen1, John Golbeck1, 2, 1 Department of Biochemistry and Molecular Biology, USA2 Department of Chemistry, University Park, PA, USA (P2A-13) Quantitative molecular volume changes of photoreactions in photosynthesis and visual system revealed by pulsed photoacoustic spectroscopy. (Abstract 193) Harvey Jian-Min Hou*,1, Thomas Sakmar2, David Mauzerall2, 1 Department of Chemistry, Gonzaga University, Spokane, WA, USA2 The Rockefeller University, New York, NY, USA (P2A-14) Characterization of Photosystem I complex purified from a diatom, Chaetoceros gracilis. (Abstract 194) Yohei Ikeda*,1, Kazuhiko Satoh1, Yasuhiro Kashino1, 1 Himeji Institute of Technology, Faculty of Science, Hyogo, 678-1297, Japan (P2A-15) Purification of two ferredoxins and cloning their genes from the photosynthetic bacterium Heliobacillus mobilis. (Abstract 195) Atsushi Hatano1, Daisuke Seo2, Masaharu Kitashima*,1, Hidehiro Sakurai2, Kazuhito Inoue1, 1 Dept. Biol. Sci., Hiratsuka, Kanagawa, Japan2 Dept. Biol., Shinjuku, Tokyo, Japan (P2A-16) Delicate distinction between absorption spectra of bacteriochlorophyll epimers. (Abstract 196) Kuniyuki TAKAHASHI1, Yuka ITOH1, Machiko AKIYAMA1, Tadashi WATANABE2, Kazuhito INOUE3, Toru OBA4, Mitsuo UMETSU5, Masami KOBAYASHI*,1, 1 Inst. Materials Sci., Univ. Tsukuba, Japan2 Inst. Industrial Sci., Univ. Tokyo, Japan3 Dept. Biol. Sci., Kanagawa Univ., Japan4 Dept. Appl. Chem., Utsunomiya Univ, Japan5 Inst. Multi. Res. Adv. Mat., Tohoku Univ., Japan (P2A-17) Energy transfer and charge separation dynamics in chlorophyll d-based Photosystem I from Acaryochloris marina. (Abstract 197) Shigeichi Kumazaki*,1, Isamu Ikegami2, Shigeru Itoh3, 1 Department of Chemistry, Graduate School of Science, Kyoto, Japan2 Faculty of Pharmaceutical Sciences, Sagamiko, Kanagawa, Japan3 Department of Physics, Graduate School of Science, Nagoya, Japan (P2A-18) Directionality of electron transport through Photosystem I of Chlamydomonas reinhardtii probed by transient electron paramagnetic resonance. (Abstract 198) Michael McConnell*,1, V.M. Ramesh1, Ingelög Wyndhamn2, Art van der Est2, Andrew Webber1, 1 School of Life Sciences and Center for the Study of Early Events in Photosynthesis, Tempe, Arizona, USA2 Department of Chemistry, St. Catharines, Ontario, Canada (P2A-19) Excited state dynamics in the PSI-LHCI supercomplex from Chlamydomonas reinhardtii: excitation wavelength dependence study. (Abstract 199) Alexander Melkozernov*,1, Joanna Kargul2, Su Lin1, James Barber2, Robert Blankenship1, 1 Department of Chemistry and Biochemistry, Tempe, AZ, USA2 Wolfson Laboratories, Department of Biological Sciences, London, UK (P2A-20) Functional characterization of the evolutionarily divergent fern plastocyanin. (Abstract 200) José Navarro*,1, Christian Lowe2, Reinout Amons3, Takamitsu Kohzuma4, Gerard Canters2, Miguel de la Rosa1, Marcellus Ubbink2, Manuel Hervás1, 1 Instituto de Bioquímica Vegetal y Fotosíntesis, Sevilla, Sevilla, Spain2 Leiden Institute of Chemistry, Leiden, The Netherlands3 Department of Molecular Cell Biology, Leiden, The Netherlands4 Department of Chemistry, Mito, Japan (P2A-21) The requirement of carotenoids for the stable assembly of the photosynthetic complexes. (Abstract 201) Kulsam Ali1, Michael Evans1, Saul Purton*,1, 1 Department of Biology, London, United Kingdom (P2A-22) Asymmetric hydrogen bonding between the quinone electron acceptor and the protein in Photosystem I as studied by various EPR methods. (Abstract 202) Yulia Pushkar*,1, Herbert Zimmermann2, Dietmar Stehlik1, 1 Institut für Experimentalphysik, Freie Universität Berlin, Berlin, Germany2 Max-Planck Institut für Medizinische Forschung, Jahnstrasse 29, Heidelberg, Germany (P2A-23) The role of the asymmetric protein environment upon the properties of P700. (Abstract 203) Kevin Redding*,1, Maria Pantelidou2, Tatyana Konovalova1, Johan van Tol3, Fabrice Rappaport4, Gary Hastings5, Brian Abbott1, Alexander Petrenko1, 1 The University of Alabama, Tuscaloosa, Alabama, USA2 Iowa State University, Ames, Iowa, USA3 National High Magnetic Field Laboratory, Tallahassee, Florida, USA4 Institut de Biologie Physico-Chimique, Paris, France5 Georgia State University, Atlanta, GA, USA (P2A-24) Photochemically induced dynamic nuclear polarization in Photosystem I observed by 13C magic-angle spinning NMR. (Abstract 204) Esha Roy*,1, Alia1, Peter Gast2, Hans van Gorkom2, Huub de Groot1, Gunnar Jeschke3, Jörg Matysik1, 1 Leiden Institute of Chemistry, Leiden, The Netherlands2 Department of Biophysics, Leiden, The Netherlands3 Max-Planck-Institut für Polymerforschung, Mainz, Germany (P2A-25) A new model for electron transfer in PSI: equilibrium between FX and both phylloquinones. (Abstract 205) Stefano Santabarbara*,1, Peter Heathcote1, Micheal Evans2, 1 School of Biological Sciences, Queen Mary University of London, London, United Kingdom2 Department of Biology, University College London, United kingdom (P2A-26) Spectral properties of the PS I reaction center from T. elongatus: structure-based modelling of optical spectra. (Abstract 206) Eberhard Schlodder*,1, Enrica Bordignon2, Thomas Renger3, 1 Max-Volmer-Laboratorium für Biophysikalische Chemie, Berlin, Germany2 Universität Osnabrück, Osnabrück, Germany3 Institut für Chemie (Kristallographie), Berlin, Germany (P2A-27) Functional genomics of genes for biogenesis of Fe/S proteins in cyanobacteria. (Abstract 207) Gaozhong Shen*,1, Ramakrishnan Balasubramanian1, Tao Wang1, Bhramara Tirupati1, Martin Bollinger1, 2, John Golbeck1, 2, Donald Bryant1, 1 Department of Biochemistry & Molecular Biology, University Park, PA, USA2 Department of Chemistry, University Park, PA, USA (P2A-28) The energy gap between P700+-A0- and P700* in Photosystem I determined by delayed fluorescence at 270-77 K. (Abstract 208) Yutaka Shibata*,1, Takashi Kasahara1, Sinpei Akai1, Shigeru Itoh1, Isamu Ikegami2, 1 School of Material Science (Physics), Nagoya, Japan2 Teikyo University, School of Pharmaceutical Sciences, Tsukui-gun, Kanagawa, Japan (P2A-29) Structural analysis of PSI-LHC supercomplexes of state I and state I forms from Chlamydomonas reinhardtii. (Abstract 209) Rajagopal Subramanyam*,1, 3, Ingo Grotjohann2, 3, Jean-David Rochaix4, Petra Fromme2, 3, Andrew Webber1, 3, 1 School of Life Sciences, Arizona State University, Tempe, Arizona, USA3 Center for the Study of Early Events in Photosynthesis, Arizona State University, Tempe, Arizona, USA2 Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona, USA4 Departments of Molecular Biology and Plant Biology, University of Geneva,12111 Geneva 4, Switzerland (P2A-30) Excitation-energy trapping to P700 from core-antenna pigments including red Chls in Photosystem I. (Abstract 210) Hitoshi SUMI*,, Koichiro MUKAI1, 1 Institute of Materials Science, Tsukuba, Ibaraki-ken, Japan (P2A-31) The unique properties of Photosystem I of a cyanobacterium Gloeobacter violaceus PCC7421. (Abstract 211) Hidetoshi Inoue1, Tohru Tsuchiya*,1, 2, Soichirou Stoh3, Hideaki Miyashita1, 2, Ayumi Tanaka3, Mamoru Mimuro1, 2, 1 Graduate School of Human and Environmental Studies, Japan2 Department of Technology and Ecology, Japan3 The Institute of Low Temperature Science, Japan (P2A-32) The function of the PscD subunit of the photosynthetic reaction center complex in Chlorobium tepidum. (Abstract 212) Yusuke Tsukatani*,1, Shigeru Itoh2, Ryo Miyamoto2, Hirozo Oh-oka1, 1 Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka, Japan2 School of Material Science (Physics), Graduate School of Science, Nagoya University, Nagoya, Aichi, Japan (P2A-33) Alteration of phenylalanine F669(PsaB) slows electron transfer in the PsaA branch of Photosystem I. (Abstract 213) Art van der Est*,1, Yuri Kandrashkin1, John Golbeck2, 1 Dept. of Chemistry, Brock University, St Catharines, Ontario, Canada2 Dept. of Biochemistry and Molecular Biology, University Park, PA, USA (P2A-34) Evidence for bi-directional electron transfer in Chlamydomonas PSI: analysis of mutants of the axial ligands to Chl A0. (Abstract 214) Velupillai Mani Ramesh*,1, Krzysztof Gibasiewicz, Su Lin, Scott Bingham1, Andrew Webber1, 1 School of Life Sciences,Center for the Study of Early Events in Photosynthesis, Tempe, AZ, USA (P2A-35) Computed infrared spectra of protonated and metal-bound methylimidazole. (Abstract 215) Ruili Wang*,1, Gary Hastings*,1, 1 Department of Physics and Astronomy, Atlanta, GA, USA (P2A-36) Electron transfer along both branches of excitonically coupled chlorophylls in PSI. (Abstract 216) Andrew Webber*,1, V Ramesh1, Krzysztof Gibasiewicz3, Su Lin1, Neal Woodbury1, 1 School of Life Sciences, Tempe, AZ, USA3 Department of Physics, Poland (P2A-37) Photosynthetic electron transport supported by methyl viologen in the obligate shade plants Panax ginseng and Panax quinquefolius. (Abstract 217) Matthew Woods*,1, Graeme Parmenter2, Julie-Ann Miskell1, Julian Eaton-Rye1, 1 Biochemistry Department, Dunedin, New Zealand2 New Zealand Institute for Crop and Food Research Ltd, Mosgeil, New Zealand. (P2A-38) Low-temperature electron transfer in A0 binding site point mutants studied using transient EPR and CW-EPR. (Abstract 218) Ingelög Wyndhamn*,1, Art van der Est1, Rachel Cohen2, John Golbeck2, 1 Department of Chemistry, Brock University, 500 Glenridge Avenue, St.Catharines, ON, CANADA2
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