PARENT SESSION
Posters P5A Type II reaction centres : Structure. Abstracts (289-312)

Characterization of thylakoid membranes isolated from different plant species. Annette Friebe^*,1, Carmen Schulten², Andreas Brandt¹, Heide Schnabl², ¹ center of advanced european studies and research, Bonn, Germany² Institute of Molecular Physiology and Biotechnology of Plants, Bonn, Germany

ABSTRACT- This research is focused on the isolation of D1-protein for further investigation of interaction with phytotoxic compounds and development of new biosensor concepts. Therefore, thylakoid membranes have been isolated from Vicia faba, Pisum sativum, Lactuca sativa and Spinacia oleracea. Isolated membranes have been characterized using 2D-gel electrophoresis (green native- and SDS-Page) and Western blots with a polyclonal anti D1-protein antibody and have been compared for the different plant species. The activity of the photosynthetic electron transport was evaluated by means of pulse amplitude modulated fluorescence measurements. The results are discussed with respect to the application of different isolation protocols. Further fractionation of the thylakoid membranes was performed using solvent partition and chromatographic procedures including fast protein liquid chromatography (FPLC). Results of the biochemical characterization of selected fractions are presented.

KEY WORDS: thylakoid membrane, protein interaction, D1-protein, type II reaction centers