PARENT SESSION
Posters P3B Photosynthetic acclimation: Ecophysiology, diverse environments. Abstracts (479-522)

Lowered stomata conductance promotes the oxidative burst, an essential factor in the promotion of PCD. Per Mühlenbock^*,1, Stanislaw Karpinski¹, ¹ Department of Botany, Stockholm University, Lilla Frescativ. 5, SE-10691, Stockholm., Sweden

ABSTRACT- The role of stomata in photosynthesis and their importance in plant productivity is well established and a number of studies have shown their importance for the plants interaction with the abiotic environment. However an increasing amount of evidence is pointing toward an important role for stomata also in biotic defense responses. Using PCD mutants and pathogen infection we set out to investigate the role of stomata in biotic stress. Physiological characterization of the lsd1 mutant of Arabidopsis thaliana revealed a correlation between stomata conductance, H₂O₂ production and the spreading of cell death. When challenging wild type plants with the bacterial pathogen Pseudomonas syringae, stomata closed and limiting gas exchange strongly enhanced the spreading of cell death both in infected plants and in the _lsd1 mutant. Further studies showed that limiting gas exchange enhances the production of ROS that lead to the formation of focused HR like lesions in ws-0. Our results clearly indicate the importance of stomata regulation in the spreading of cell death.

KEY WORDS: Stomata conductance, Oxidative stress, Pathogen infection