PARENT SESSION
Posters P6B Photosynthetic acclimation: Mechanisms and gene expression. Abstracts (531-578)

The expression profile of rice genes in planta related to the protection and regulation mechanisms against abiotic stress in the light. Ismayil Zulfugarov^*,1, Byoung Chull Chung², Hong Jin Hwang³, Choong-Hwan Ryu⁴, Byoung Yong Moon⁵, Gynheung An⁶, Choon-Hwan Lee⁷, ¹ Department of Molecular Biology, Pusan National University, Busan, Republic of Korea² Department of Molecular Biology, Pusan National University, Busan, Republic of Korea³ Department of Molecular Biology, Pusan National University, Busan, Republic of Korea⁴ Department of Life Science, Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea⁵ Department of Biology, Inje University, Gimhae, Republic of Korea⁶ 2Department of Life Science, Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea⁷ Department of Molecular Biology, Pusan National University, Busan, Republic of Korea

ABSTRACT- The expression profile of genes related to protection and regulation mechanisms against photoinhibition and photooxidation of photosynthetic apparatus of the rice plant Oryza sativa L were studied by using promoter-trap T-DNA insertion lines generated with a binary T-DNA vector, pGA2717, that contains the promoter-less green fluorescence protein gene next to LB of the T-DNA. Leaf discs and stems from 9,000 promoter-trap lines were put in 96-well plates and treated with 13 different abiotic stresses. GFP fluorescence was measured using a homemade image capture system using argon laser as excitation beam at 488 nm. Each image of 96 samples in a plate was analyzed by homemade software used for microarray analysis. The analysis starts with a normalization step for the distribution of light intensities, and next steps until clustering are similar to those used for microarray analysis. From leaf samples treated with 6 different stress, we could obtain 250 lines belong to 16 different clusters. From the whole analysis, we could obtain total 792 lines showing positive GFP signals under 13 stress conditions. In addition, the GFP fluorescence from 3212 lines of T2 seeds were tracked non-destructively during their development stages. We selected a total of 74 lines which included 14 of their mature seeds, 51 of the three-day-old etiolated seedling, 9 of seedlings after greening and 3 of seedlings treated with abscisic acid and 3 lines were present more than once case. Among the lines with positive signals at both T1 and T2 generations, the genomic sequences flanking T-DNA of 208 lines were isolated. T-DNA was inserted to genic region in 89 lines and 37 lines were putative GFP. All the confirmation tests for T2 generation were passed, but the remaining test for T1 generation experiments remains for reconfirmation. The possibility of using this trap lines for isolating genes with different stress-inducible promoter activities will be discussed.

KEY WORDS: gene regulation, abiotic stress, photosynthesis, GFP