PARENT SESSION
Posters P5D Emerging techniques and systems. Abstracts (731-741)

Photosynthetic biosensor and molecularly imprinted polymer. Florent Breton¹, Pierre Euzet¹, Aurelie Jacquemond¹, Sergey Pilesky², Maria-Teresa Giardi³, Regis Rouillon^*,1, ¹ Université de Perpignan, Equipe Biomem, Perpignan, France^{2 3}

ABSTRACT- Herbicides acting as inhibitors of photosystem II are still widely used in agriculture, since they provide a low-cost weed control. They include principally triazines, phenylueras, triazinones. These products have a common mode of action: the ability to interact with the D1 protein by replacing the plastoquinone, which is a secondary electron acceptor of PSII reaction centre (QB) from its binding site. Photosynthetic biosensors allow to detect these pollutants in the environment but compared to sophisticated and costly methods used in the laboratory, their sensibility and their selectivity are insufficient. A molecularly imprinted polymer (MIP) with cyanazine as template was developed and used as sorbent for solid phase extraction (SPE) before the measure of the photosynthetic activity (fluorescence). With this MIP cartridge, it was possible to concentrate the herbicides, which were present in different samples, and to increase the sensibility of the photosynthetic biosensor. In comparison with a normal SPE cartridge, it was also possible to increase the selectivity of the photosynthetic material. The MIP with the cyanazine as template allowed to do separation between triazines (cyanazine, atrazine) which were retain into the cartridge and a phenylurea (diuron) or a triazinone (metribuzin) which were found in the washing solvent.

KEY WORDS: Photosynthetic biosensor, Molecularly imprinted polymer, Herbicides, Solid phase extraction