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Work-in-Progress
(WIP-382) Comparison of radiation-induced changes in blood antioxidants between radiation-resistant C3H and radiation-sensitive C57BL/6 mice.
Cho, Woo-Hyong1, Park, Ji-Sun1, Park, Eun-Mi1, Park, Young-Mee*,1, 1 Division of Chemistry and Biology, Incheon, Korea
ABSTRACT-
The role of antioxidative defense systems on the strain difference in radiation sensitivity was investigated by comparing radiation-induced changes in blood antioxidants between radiation-resistant C3H and radiation-sensitive C57BL/6 mice. Glutathione (GSH) content in red blood cells (RBCs) decreased upon irradiation at a single 10 Gy dose, and the rate of decrease was greater in C57BL/6. RBCs of radiation-resistant C3H showed higher basal activities antioxidant enzymes, glutathione peroxidase (Gpx), superoxide dismutase (SOD) and glucose 6-phosphate dehydrogenase (G6PD), than radiation-sensitive C57BL/6 mice, and radiation caused a transient increase in enzyme activities in C3H mice. On the other hand, with an exception of glutathione peroxidase which showed a transient increase, enzyme activities in RBCs of C57BL/6 mice showed gradual decrease upon irradiation. Contrary to the RBCs, the activities of plasma antioxidant enzymes, Gpx, SOD and glutathione S-transferase (GST), were higher in C57BL/6 than C3H mice. Radiation caused rather persisting increase in the activities and the degree of activation was higher in C57BL/6. The results suggest that depletion of glutathione and increase of antioxidant enzyme activities are indicative of the degree of oxidative stress caused by radiation, and therefore, the activities of blood antioxidant systems may serve as indicators of difference in radiation sensitivity. [Supported by grants from MOST]
KEYWORDS: Radiation, Red blood cells (RBCs), Radioresistant/radiosensitive, Antioxidants