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PARENT SESSION

Mutagenesis/Clastogenesis/Carcinogenesis

Sunday, October 16, 2005 3:00 PM-5:00 PM Exhibit Hall

(PP076) The level of clastogenic factors as marker of mutation pressure in human population.

Marozik, Pavel*,1, Marozik, Mikhail2, Melnov, Sergei2, 1 Radiation and Environmental Science Centre, Dublin, Dublin, Ireland2 Human Biology, Minsk, Minsk, Belarus

ABSTRACT- Clastogenic factors (CFs) were first described in the plasma of persons who had been irradiated accidentally or therapeutically. But they are not induced only by radiation. CF are circulating in plasma of patients with different pathological conditions accompanied by oxidative stress. CFs are a mixture of prooxidants with chromosome damaging properties. The formation and damaging action of CFs is mediated through superoxide anions. We studied the level of CF formation in blood serum of Chernobyl liquidators 1986-1987 (acute irradiation), people working in territories contaminated after Chernobyl accident (chronic irradiation), liver cirrhosis patients and patients with acute virus infection. Blood serum was extracted using serum separation tubes (Vacutainers), filtered and then frozen and stored at -20 C. The serum was tested on the HPV-G cell line. Micronuclei frequency as an indicator of serum influence has been used. People exposed to chronic irradiation have an increased mutagenic pressure, expressed as a considerable increase of micronuclei frequency compared with controls. The same result without significant difference was observed in people exposed to an acute dose of radiation. These results indicate that radiation exposure increases the level of CF accumulation. The level of micronuclei induction of the patients with liver cirrhosis was higher than in controls, but much lower than in patients exposed to radiation. And the level of micronuclei from the patients with acute virus infection in the active stage was higher than in all previous cases, possibly the result of extremely high levels of oxidants. The results show that the level of clastogenic activity may correspond to the level of activity of pathological processes and to radiation influence and can be fixed even 15 years after radiation exposure. Oxidative stress may substantially influence the level of CF, creating a possibility of temporary destabilisation of the cell genome.

Key words: clastogenic factors, Chernobyl


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2005 RRS