Genomic Maintenance & Repair

Monday, October 17, 2005 3:00 PM-5:00 PM Exhibit Hall

(PP301) Identification of BISET1, a BCCIP-Interacting SET domain protein.

Fan, Jinjiang*,1, Shen, Zhiyuan1, 1 Dept. of Molecular Genetics and Microbiology, Albuquerque, NM, USA

ABSTRACT- BCCIP is a BRCA2 and CDKN1A (p21/Cip1) Interacting Protein, which plays a significant role in DNA repair and cell cycle checkpoint control. To further understand the mechanisms by which BCCIP regulates DNA repair and cell cycle control, we sought to identify proteins that may cooperate with BCCIP. Using a bioinformatics approach, we identified a previously un-characterized S. cerevisiae protein YPL208W that interacts with BCP1, the yeast homologue of mammalian BCCIP. We then cloned the human homologue of YPL208W, and confirmed its interaction with human BCCIP by co-immunoprecipitation. Because the human homologue of YPL208W contains a characteristic SET domain, it is tentatively named BISET1 for BCCIP-Interacting SET domain protein 1. The BISET1 gene is located on chromosome 21q22.12. BISET1 is a nuclear protein that has six major isoforms, which are the products of alternative splicing. The SET-domain proteins are a family of protein (including histone) methyl-transferases that functions in chromatin remodeling, DNA repair, and transcription. The interaction between BISET1 and BCCIP suggests a role of the BCCIP-dependent molecular pathway in chromatin remodeling and DNA damage response.

Key words: BCCIP, SET domain, BISET1, chromatin remodeling

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2005 RRS