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(PP065) M-BAND analysis of chromosome aberrations induced by heavy ion radiations in human epithelia cells.
Hada, Megumi*,1, 2, Desai, Nirav2, 3, Cucinotta, Francis2, Gonda, Steve2, Wu, Honglu2, 1 Division of Space Life Science, Houston, TX, USA2 Human Adaptation and Countermeasures Office, Houston, TX, USA3 Radiation Biology, Houston, TX, USA
ABSTRACT- Energetic heavy ions pose a great health risk to astronauts in extended ISS and future Mars missions. High-LET heavy ions are particularly effective in causing various biological effects, including cell inactivation, genetic mutations and cancer induction. Most of these biological endpoints are closely related to chromosomal damage, which can be utilized as a biomarker for radiation insults. Previously, we had studied chromosome aberrations in human lymphocytes and fibroblasts induced by both low- and high-LET radiation using FISH and multicolor fluorescence in situ hybridization (mFISH) techniques. In this study, we exposed human epithelia cells in vitro to gamma rays and energetic particles of varying types and energies and dose rates, and analyzed chromosomal damages using the multicolor banding in situ hybridization (mBAND) procedure. Confluent human epithelia cells (CH184B5F5/M10) were exposed to energetic heavy ions at NASA Space Radiation Laboratory (NSRL) at the Brookhaven National Laboratory or Cs-gamma radiation source at the Baylor College. After colcemid and Calyculin A treatment, cells were fixed and painted with XCyte3 mBAND kit (MetaSystems) and chromosome aberrations were analyzed with mBAND analysis system (MetaSystems). With this technique, individually painted chromosomal bands on one chromosome allowed the identification of interchromosomal aberrations (translocation to unpainted chromosomes) and intrachromosomal aberrations (inversions and deletions within a single painted chromosome). The possible relationship between the frequency of inter- and intra-chromosomal exchanges and the track structure of radiation is discussed. The work was supported by the NASA Space Radiation Health Program.
Key words: Chromosome aberration, High-LET radiation, m-BAND, Human epithelia cell
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