Sunday, October 16, 2005 3:00 PM-5:00 PM Exhibit Hall

(PP088) DNA damage induced by combined cadmium and radiation exposure in vitro and in cultured medaka fish cells.

Grygoryev, Dmytro1, Moskalenko, Oleksandr1, Zimbrick, John*,1, 1 Department of Environmental and Radiological Health Sciences, Fort Collins, CO, USA

ABSTRACT- It is known that the formation of ionizing radiation-induced DNA damage is modulated by a number of factors such as heavy metal ions. Many of these ions are cytotoxic and carcinogenic. Cadmium (Cd) is a toxic transitional metal that has been recognized as a carcinogen by the International Agency for Research on Cancer. It can affect the activity of cellular DNA repair systems. The biological effects of cadmium are cumulative. We studied the formation of DNA base damage and strand breaks caused by the simultaneous action of cadmium and gamma radiation. The studies were performed in vitro (aqueous DNA solution) and in vivo using medaka fish (Oryzias latipes) as well as medaka embryonic cell cultures. It was found that Cd treatment alone did not produce measureable DNA damage in salmon sperm DNA in vitro. When Cd was added to the DNA solutions and combined with radiation its presence did not significantly change the amount of damage induced by radiation alone. The results are different when medaka cells are irradiated in the presence or absence of Cd. Under some conditions it was found that the amount of DNA base damage decreased in the treated versus the control cells.

Key words: DNA damage, cadmium, radiation

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2005 RRS