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Experimental and Clinical Therapeutics

Monday, October 17, 2005 3:00 PM-5:00 PM Exhibit Hall

(PP192) Low dose ionizing radiation does not affect the expression of 1 integrin and EGF receptor in DU-145 and PC3 prostate cancer cells.

Wang, Tao*,1,2, Languino, Lucia2,3, FitzGerald, Thomas1,4, 1 Univ. of Massachusetts Medical School, Worcester, MA, United States2 Univ. of Massachusetts Medical School, Worcester, MA, United States3 Univ. of Massachusetts Medical School, Worcester, MA, United States4 Univ. of Massachusetts Medical School, Worcester, MA, United States

ABSTRACT- Purpose/Objective: Our previous data demonstrated that high dose fractionated ionizing radiation inhibits the expression of 1 integrin in prostate cancer cells(Simon et al., 2005, Prostate). In this study, we investigated the expression of epidermal growth factor receptors (EGFR) and 1 integrin in prostate cancer cells exposed to the low dose ionizing radiation. The adhesion of both cell lines to extracellular matrix components (ECM) was studied to explore the biological role of integrins and possible role of EGF in the integrin function in cells exposed to radiation. Materials/Methods: DU-145 and PC3 prostate cancer cells were given x-ray at a single dose fashion with doses up to 8 Gy. After 24 or 48 hrs of radiation, cells were collected and analyzed using immunoblotting and FACS analysis to determine the expression of EGFR and 1 integrin. Adhesion assays were performed to see if the expression of functional 1 integrin correlates with changes in interaction between cells and fibronectin. Results: 1) Exposure to 4 or 8 Gy did not affect integrin and EGFR expression in cells as detected by immunobloting. 2) Radiation at 4 or 8 Gy did not affect cell adhesion to fibronectin. 3) No obvious changes in EGFR and 1 integrin expression on cell surface was detected; 4) Within 2 hours post radiation, EGFR was not activated in cells exposed to 2 and 4 Gy as detected using the antibody specific for the EGFR phosphorylated form. Conclusions: Low dose ionizing radiation does not have an effect on the expression of EGFR and 1 integrins on DU-145 or PC3 cells. These data imply that integrin based membrane and EGFR based therapies should be active and effective during the initial phases of chemo-radiation management.

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2005 RRS