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(195) Toxicokinetics and biotransformation of p-nitrophenol in red abalone (Haliotis rufescens).
TenBrook, Patti*,1, Kendall, Shellie1, Viant, Mark1, Tjeerdema, Ronald1, 1 University of California, Davis, CA, USA
ABSTRACT- Red abalone (Haliotis rufescens) were exposed to 3.6 mM (0.5 ppm) 14C-labelled p-nitrophenol (PNP) for 24 h, then were allowed to depurate in clean seawater for another 24 h. Absorption, conditional uptake clearance and elimination rate constants were 0.12 + 0.04 h-1, 3.2 + 1.1 mL g-1 h-1 and 0.05 + 0.02 h-1, respectively. The sigmoidal shape of the PNP uptake curve suggests a biphasic process. A whole-organism total concentration factor (TCF) of 2.37 + 0.07 was determined from equilibrium tissue and water concentrations, with the highest concentration of PNP plus metabolites found in gill tissue (11.8 + 0.2 nmol g-1, wet weight) . Digestive gland, foot muscle and remaining body tissues accumulated 8.8 + 0.9, 7.7 + 0.6 and 7.5 + 0.6 nmol g-1 radiolabelled residues, respectively. Abalone depurated 87% of absorbed PNP within 24 h, of which 87.5 + 3.1% was unmetabolized parent compound, 13.1 + 3.1% was p-nitrophenylsulfate, 0.32 + 0.09% was p-nitroanisole, and 0.14 + 0.07% was p-acetamidophenol.
Key words: p-nitrophenol, abalone, toxicokinetics, biotransformation
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