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(P174) Assessment of PAH Bioavailability in soil to Eisenia fetida using Solid Phase Microextraction Technology.
Tost, Brian*,1, Lanno, Roman1, Booth, Lynn1, Booth, Phil1, Basta, Nick2, 1 Ohio State University, Columbus, Ohio, USA2 Oklahoma State University, Stillwater, Oklahoma, USA
ABSTRACT- A fast, accurate, and inexpensive method for assessing exposure to the potentially bioavailable fraction of hydrophobic contaminants, such as polynuclear aromatic hydrocarbons (PAHs), is desired for applications in both laboratory and field ecotoxicology. Solid Phase Microextraction (SPME) fibers provide a technology that demonstrates all of these qualities, including the fact that little to no hazardous waste is generated from the procedure. The bioavailability of pyrene to the manure worm, Eisenia fetida, in Kirkland (pH = 5.78, Organic Carbon % = 0.66, Clay Content % = 27.5), Perkins (pH = 3.80, Organic Carbon % = 0.72, Clay Content % = 12.3), and Webster (pH = 5.46, Organic Carbon % = 2.4, Clay Content % = 35.6) soils was evaluated using SPME fibers (Supelco Brand, 70 mm Carbowax/DVB (Divinylbenzene)). In addition to SPME, C18 extraction disks (3M Empore High Performance Extraction Disks, C18 (octadecyl), 47 mm) were also used to evaluate the use of other passive sampling devices (PSDs) to evaluate the bioavailable PAH fraction. Parallel extractions were performed on soil and earthworms using the Soxhlet continuous solid-liquid extraction technique to compare bioavailability, and to assess the viability of SPME and C18 disks as acceptable substitutes for Soxhlet extraction. The results of this study will provide information on the effects of the soil type on the bioavailability of pyrene.
Key words: eisenia fetida, solid phase microextraction, pahs, bioavailability
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