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(P651) Organochlorine Residues in Complete Clutches of Morelet's Crocodile (Crocodylus moreletii) Eggs.
Wu, Ted*,1,2, Caņas, Jaclyn2, Thomas, Rainwater2, Platt, Steve3, McMurry, Scott2, Anderson, Todd2, 1 University of Alaska at Fairbanks, Fairbanks, AK, USA2 The Institute of Environmental and Human Health, Texas Tech University, Lubbock, TX, USA3 Wildlife Conservation Society, Bronx, NY, USA
ABSTRACT- Here, seven complete clutches of Morelet's crocodile (Crocodylus moreletii) eggs were obtained in northern Belize and examined for OC pesticide residues. Eggs were extracted using a Dionex 200 Accelerated Solvent Extractor and analyzed by GC/ECD. The primary OC detected, p,p-DDE, was found in every egg analyzed (n = 175). Other OCs detected included p,p-DDT, p,p-DDD, methoxychlor, and aldrin. Concentrations of individual OCs (wet weight) ranged from 4 ppb (ng chemical/g egg) to >0.5 ppm (g chemical/g egg). Total concentration of OCs (sum of all OCs) for one egg collected from a nest at Gold Button Lagoon (GB) reached as high as 0.7 ppm. A statistical evaluation of DDE levels in three complete clutches was used to derive the minimum number of eggs needed from a clutch to accurately determine the mean DDE concentration representative of that clutch. Sample sizes of 8 eggs (80% confidence interval) and 11 eggs (90% confidence level) were determined to yield an accurate estimate of contaminant levels in a full clutch of eggs based on an average standard deviation (0.26 ng/g), average clutch size (n = 20), and acceptable deviation from the mean (± 0.1). The statistically recommended sample size of 11 eggs at t(90%) was successfully tested on four additional clutches.
Key words: complete clutch, dde, crocodylus moreletii, eggs
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