PARENT SESSION
PT2 - Endocrine Disruption
Tuesday, 19 November 2002
8:00 AM to 6:30 PM
Exhibit Hall
(P567) Specific distribution of organotin compounds and endocrine disruption in gastropods observed in the inshore ecosystem.
Horiguchi, Toshihiro*,1, Matsuo, Taiki2, Cho, Hyeon-Seo3, Kaya, Miyuki1, Shiraishi, Hiroaki1, Morita, Masatoshi1, Adachi, Yoshikazu2, 1 National Institute for Environmental Studies, Tsukuba, Ibaraki, Japan2 Ibaraki University, Inashiki, Ibaraki, Japan3 Yosu National University, Yosu, Chonnam, Korea
ABSTRACT- Environmental samples, such as seawater, bottom sediment, algae, fish and shellfish, were collected to know contamination levels by organotin (butyltin and phenyltin) compounds and specific distribution of those compounds in inshore ecosystems of Jogashima, Kanagawa Prefecture in September 1996. Organotin compounds were extracted, using 1N HBr/ethanol and 0.1% tropolone/benzene, and then derivatized with propylmagnesium bromide. Organotin compounds in the sample were determined by GC-FPD after cleaning up by silica gel column chromatography. Before chemical analysis, anatomical observation and microscopic histological examination for gastropods (Archaeogastropoda, Mesogastropoda and Neogastropoda) were carried out to evaluate the degree of masculinization of females, such as imposex. Higher concentrations of tributyltin (TBT) and triphenyltin (TPhT) were detected in seawater and bottom sediment around shipyards located in Jogashima. Tissue concentrations of TPhT seemed to get higher along the food chain of marine organisms, while it was unclear in tissue concentrations of TBT. Regarding tissue distribution of TPhT, higher concentrations were observed in liver (digestive gland), kidney and brain (the head including ganglia), while higher TBT concentrations were detected in gill (ctenidium), heart and kidney. Accumulation pattern of TBT and TPhT in tissue of Mugil cephalus cephalus was different from that of other organisms. Severely affected stages of imposex were observed in both Mesogastropoda and Neogastropoda, such as Monoplex parthenopewn echo (sterile) and Thais clavigera (sterile and sex change), respectively. Ovarian spermatogenesis was also observed in abalone, Haliotis madaka and H. gigantea. Severe contamination by TBT and TPhT near shipyards could have caused endocrine disruption in gastropods, including abalone, as well as remarkable accumulation of those compounds in marine organisms from Jogashima.
Key words: specific distribution, tributyltin (TBT), triphenyltin (TPhT), gastropod endocrine disruption