PARENT SESSION
PT2 - Endocrine Disruption
Tuesday, 19 November 2002
8:00 AM to 6:30 PM
Exhibit Hall

(P541) DETAILED REVIEW PAPER FOR FISH REPRODUCTIVE SCREENING ASSAYS.

Schultz, Irvin^*,1, Skillman, Ann¹, Blanton, Michael¹, Nagler, James², ¹ Battelle PND, Sequim, WA, USA² University of Idaho, Moscow, ID, USA

ABSTRACT- We prepared a Detailed Review Paper (DRP) for fish reproductive screening assays with endocrine endpoints for the U.S. Environmental Protection Agency. The DRP summarizes, explains, and documents relevant principles, methods, and techniques recommended for development of an initial protocol(s), and will identify those issues that may require prevalidation studies to adequately address. The focus of the DRP will be on the relevance and sensitivity of the candidate assays/endpoints for identifying chemicals that affect the endocrine system in fathead minnows, zebrafish, and Japanese medaka. The DRP will provide a basis for selecting the most promising methods for assessing chemical effects on endocrine systems in fish and for determining future studies required to resolve outstanding issues relative to method development, including the need for additional targeted prevalidation studies. This information will be used to support the design of a draft protocol for use in the validation phase of protocol design, with the ultimate goal of developing future EPA/OECD test method guidelines. The findings from the DRP efforts will be presented.

Key words: Endocrine, Disruptors, Fathead , Minnow