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(142) Assessment of Exposure of Cattle to Oil Field Wastes.
Johnson, C. Ian*,1, Birkholz, Detlef1, 1 Enviro-Test Laboratories, Edmonton, Alberta, Canada
ABSTRACT- Exposure of cattle to oil field wastes containing crude oils and petroleum derived product is becoming recognized as a significant problem in Alberta. Recent exposure of animals to the wastes can be readily demonstrated by GC-MS analysis of extracts of rumin and/or bile for specific 'families' of alkylated PAHs and their primary and secondary metabolites. GPC cleanup and fractionation by alumina column chromatgraphy yeild extracts in which alkylated PAHs are easily characterized. Metabolites are determined by analysis of actylated bile or rumen extracts initially hydrolyzed using enzymatic hydrolysis. Past exposures can be demonstrated by analysis tissues and organs for alkylated naphthalene which in the animal months after exposure. Distribution of the residues in specific organs and adipose tissue may provide some incite regarding the exposure regime.
Key words: alkylated PAHs, residue analysis, petroleum hydrocarbons, oil field wastes
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