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MP2 Amphibian Research and Monitoring
(106) The teratogenicity and mutagenicity for 5 sites near the Anniston Army Depot (ANAD).
Rayburn, J1, Wolfe, A.1, 1 Jacksonville State University, Jacksonvile, AL, USA
ABSTRACT- In order to determine whether the water samples collected from around the Anniston Army Depot (ANAD) contained potentially harmful chemicals, two biological assays and analysis of certain metals were performed. Five sites were chosen from around ANAD and water was collected from each site at five separate collection times over 2 years. The Frog Embryo Teratogenesis Assay-Xenopus (FETAX) was used to determine the developmental toxicity of the water samples and was performed on each of the samples. A microtiter mutagenicity assay utilizing Salmonella typhimurium strains TA98 and TA100 was used to determine mutagenicity of the water samples in the second year of the study. Metal determination was done on water samples using atomic absorption spectrophotometer for each of the time intervals. Four of the sites showed significant increases in mortality and all of the sites showed significant increases in malformation as well as reduction in embryo growth as compared to controls in the FETAX assay. One sample showed a significant increase in mutation rate over controls, however, mutation rate was <20%. No metals showed differences between the sites. An Albama Legacy Grant and a Faculty Research Grant from Jacksonville State University partially funded this research.
Key words: FETAX, Xenopus laevis, Mutagenicity, Salmonella typhimurium
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