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PT01 Assessing Contaminated Soil Sites
(PT012) Genotoxicity of complex chemical mixtures in soil after bioremediation.
Parr, A.1, Phillips, T.1, He, L.-Y.1, McDonald, T.1, 2, Autenrieth, R.1, 2, Donnelly, K.1, 2, 1 Texas A&M University, College of Vet. Medicine, Dept. of Vet. Anatomy and Public Health, College Station, Texas, USA2 Texas A&M University, School of Rural Public Health, Dept. of Environmental and Occupational Health, College Station, Texas, USA
ABSTRACT- One of the most cost-effective technologies for treatment of contaminated soils is bioremediation. While limited information exists to define the genotoxicity of complex environmental mixtures, even less is known regarding the potential of the products of biodegradation to produce adverse health effects. The objective of the current study was to monitor the genotoxicity of complex mixtures of polycyclic aromatic hydrocarbons(PAHs) in a land treatment unit. Soil samples were collected from a land treatment unit at a wood preserving facility in the northwestern United States. Twelve surface to 15 cm soil samples were collected annually using a 100 m X 75 m grid. Soil samples were extracted using hexane:acetone on a Tecator Soxtec extraction unit. PAHs in the extracts were quantified using GC-MS, and the genotoxicity of soils was measured using the Salmonella/microsome assay. PAH concentrations were reduced over time. The concentration of total PAHs in the soil extracts ranged from 38,000-77,000 ng/mg. The mutagenic response induced by soil extracts ranged from 81-141 net revertants per mg. There did not appear to be a correlation between benzo(a)pyrene or total PAHs and the level of mutagenicity detected in soil extracts. The results indicate that a combined testing protocol using both biological and chemical methods provides a more comprehensive view of the fate of genotoxic PAHs in soil treatment systems.
Key words: Complex Chemical Mixtures, Bioremediation, Polycyclic Aromatic Hydrocarbons, Genotoxicity
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