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PW09 Exposure and Effect Endpoints
(PW175) Differential susceptibility of fish and rat liver cells to oxidative stress and cytotoxicity upon exposure to prooxidants.
Rau, M1, Whitaker, J1, Freedman, J1, Di Guilio, R1, 1 Duke Univeristy, Durham, NC, USA
ABSTRACT- Species differences in the propensity to cope with pollutant-mediated oxidative stress can provide insight into the mechanisms behind both the mode of toxicity of a specific chemical as well as the different ways in which an organism may deal with such stressors. In this study, the effects of exposure to model prooxidants on parameters of oxidative stress were investigated in liver cells from both fish (PLHC-1) and rat (H4IIE). Cellular response to copper sulfate and menadione (both individually and in combination), and Fenton reagents (ferrous sulfate plus hydrogen peroxide) was assessed by measuring cytotoxicity, lipid peroxidation, total glutathione (GSHT), and percent glutathione disulfide (%GSSG). Additionally, transcriptional activation of an antioxidant response element (ARE) reporter gene was measured using the chloramphenicol acetyltransferase (CAT) assay in response to these chemicals. In general, the fish cells were significantly more sensitive to copper sulfate and Fenton reagent induced oxidative stress, as measured through lipid peroxidation and ARE reporter gene transcriptional activation. Copper sulfate and Fenton reagents caused a two-fold increase in %GSSG in both cell lines, whereas no consistent effects were seen with menadione exposure. Basal levels of GSHT were higher in the HII4E cells than the PLHC-1 cells, and Fenton reagents significantly reduced GSHT in fish cells but showed no effect on the rat cells. Significant differences were also observed in the cytotoxicity of the test chemicals to both cell lines, with the fish cells demonstrating a higher level of cytotoxicity. In general, lipid peroxidation and ARE transcriptional activation appeared to better reflect subsequent cytotoxicity than a change in GSHT or %GSSG. These results suggest that HII4E cells are more protected from oxidative stress than PLHC-1 cells. Additional studies are addressing oxidative stress-mediated signal transduction pathways that may play a role in the differential responses of these cells lines.
Key words: PLHC-1, oxidative stress, glutathione, lipid peroxidation
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