PH05 Fate and Effects of Energetic Compounds
Exhibit Hall
8:00 AM - Thursday
(PH039) The SCGE Study of DNA Damage on Germ Cell of Rat Induced by Nitroaromatic Compounds.
Xu, J1, 1 Northeast Normal University, Changchun, Jilin, China
ABSTRACT- Using single cell gel electrophoresis technique (SCGE) to detect DNA damage on germ cell in rat, which was induced by five tested nitroaromatic compounds. Mixed cultures of sertoli and germ cells were prepared from rat testes and their response to m-dinitrobenzene (m-DNB), 2,4-dinitrotoluene (2,4-DNT), 2,6-dinitrotoluene (2,6-DNT), 4-nitrotoluene (4-NT) and 2,4-dinitroaniline (2,4-DNAn) was studied. The results showed that all 5 chemicals had the reproductive toxicity. Every dose group and the control group were significantly different (P<0.01). All of them had led to the damage on DNA of germ cell of Kunming male rat in the definite range of concentration (m-DNB: 0.00004-0.025mmol/L; 2,4-DNT, 2,6-DNT, 4-NT: 0.000032-0.5mmol/L; 2,4-DNAn: 0.0008-0.02mmol/L). When the concentration increased, the damage rate became more obvious, which showed an evident logarithm dose-effect relationship. These observations would suggest that the COMET assay is a useful tool for examining issues related to environment stress in rat. Moreover, this paper also made a primary discussion on the safety estimate of ecological toxicology at the DNA level.
Key words: sertoli-germ cell coculture, DNA damage, nitroaromatic compounds, SCGE