PH14 Ecotoxicogenomics
Exhibit Hall
8:00 AM - Thursday
(PH172) Real time PCR based analysis of biomarker and endogenous genes expression in Japanese Medaka.
Hong, H.N.1, Park, K.S.1, Gu, M.B.1, 1 Gwangju Institute of Science and Technology, Gwangju, Korea
ABSTRACT- The expression kinetics of three biomarkers, i.e., the estrogen receptor (ER), cytochrome P450 3A(CYP3A), and p53 genes, in the Japanese Medaka liver were analyzed when this organism was exposed to nonylphenol, a known endocrine disrupting chemical. Each biomarker is representative of the estrogenic potential, steroidogenesis of chemicals, and tumor suppression, respectively. Japanese Medaka were exposed to 75 ppb, which is the LC20 as obtained from acute toxicity test, or 25 ppb for periods of 3 hrs, 12 hrs, 1 day, 2 days, 4 days and 10 days. Gene responses were quantified through the measurement of messenger RNA (mRNA) concentrations in liver extracts using a Taqman®-based real time PCR method. Using this method, the transcriptional mechanisms of each gene after nonylphenol exposure were examined. In addition, significant variations in the beta-actin expression were seen and are noteworthy since this gene and it expression is usually used as an endogenous control. Therefore, the expression patterns from several other potential endogenous control genes to various chemicals will be presented and discussed as well.
Key words: distinct expression kinetics, biomarker, Japanese Medaka, real time PCR