HA4 Ecotoxicogenomics
204 Oregon Ballroom
8:00 AM - 12:00 PM, Thursday
() Assessing the use of oligonucleotide microarrays for fathead minnow (Pimephales promelas) to examine exposure variables.
Miracle, A1, Knoebl, I1, Villeneuve, D2, Ankley, G2, Denslow, N3, Carter, B4, Larkin, P4, 1 US EPA, Ecological Exposure Research Division, Cincinnati, OH, USA2 US EPA, Mid-Continent Ecology Division, Duluth, MN, USA3 Dept. Biochemistry and Molecular Biology, U. of Florida, Gainesville, FL, USA4 EcoArray, LLC, Alachua, FL, USA
ABSTRACT- Microarray technology has proven to be a useful tool for analyzing the transcriptome of various organisms representing conditions such as disease states, developmental stages, and responses to chemical exposure. Although most commercially available arrays are limited to organisms that have complete, or near complete, genome information, efforts directed at developing arrays for other model organisms have been gaining attention in an effort to fulfill experimental and programmatic needs of academia, industry, and regulatory agencies. We describe some of the first experiments examining the use of custom made oligonucleotide arrays developed to assess transcriptome changes in a popular aquatic toxicity model, the fathead minnow (Pimephales promelas). Microarray results for adult tissues from several chemical exposures are presented. Use of guidelines following Microarray Gene Expression Data (MGED) Society standards such as MIAME (minimal information about microarray experiments) and MAGE (microarray gene expression) for experimental design and validation are described with the perspective of producing data that are useful for environmental studies. Although this work was reviewed by EPA and approved for publication, it may not necessarily reflect official Agency policy
Key words: fathead minnow, microarrays, aquatic exposure