PH05 Fate and Effects of Energetic Compounds
Exhibit Hall
8:00 AM - Thursday
(PH038) Solid phase microextraction of ADNTs in tissue.
Bowen, A.1, Condor, J.1, La Point, T.1, 1 University of North Texas, Denton, TX, USA
ABSTRACT- Solid phase microextraction (SPME) fibers have been used to measure organic compounds in environmental matrixes. In this experiment, we explored SPME extractability of 2,4,6-trinitrotoluene (TNT) metabolic products from tissue. TNT readily and predominately transforms to 2-amino-4,6-dinitrotoulene (2ADNT) and 4-amino-2,6-dinitrotoulene (4ADNT) in environmental matrixes and tissues. Tubifex tubifex (sludgeworm) was allowed to metabolize TNT into ADNTs in 24-hr static non-renewal exposure test followed by 24-hr depuration in clean reconstituted hard water. Polyacrylate-coated (PA) SPME fibers were then deployed and agitated in the tissue homogenates containing metabolically-generated ADNTs for 48 hr to provide a measure of toxicologically available ADNTs. Separate tests exposing Tubifex to ADNTs solution and exposing uncontaminated homogenized tissue to spike ADNTs were also performed to compare ADNT extractability. Extractability of metabolically-generated ADNTs from tissue homogenates was about 64% of the extractability of absorbed ADNTs and 67% of the extractability of ADNT spiked into homogenate of tissues from unexposed Tubifex . The lower SPME extractability of metabolically-generated ADNTs may be due to tissue sequestration during the TNT to ADNT metabolism.
Key words: extractability, amino-dinitrotoulene, metabolically-generated, SPME