PT04 Endocrine-Disrupting Chemicals in Wastewater Treatment Effluents
Exhibit Hall
8:00 AM - Tuesday
(PT053) Assessment of Bisphenol A estrogenic activity in water following UV and UV/H2O2 oxidation treatment.
Chen, P.-J. 1, 2, Linden, K. 1, Rosenfeldt, E. 1, Kashiwada, S. 2, Hinton , D. 2, Kullman, S. 2, 1 Department of Civil and Environmental Engineering, Duke. U., Durham, NC, USA2 Integrated Toxicology Program, Duke. U., Durham, NC, USA
ABSTRACT- Extensive studies have shown that endocrine disrupting compounds (EDCs) are present in surface water, ground water supplies and sewage effluent, causing adverse effects on human and wildlife endocrine function. UV irradiation and combined UV/H2O2 oxidation processes have been demonstrated as effective techniques for EDCs oxidation; however, the estrogenic activity of degradation products following these processes remains unknown. In this research, direct UV irradiation with low- pressure (LP) Hg UV lamps and UV combined with 10-50 ppm H2O2 were used to assess the degradation of Bisphenol A (BPA) in laboratory water. The removal rates of total estrogenic activity and the parent compound were quantitatively evaluated by in vitro Yeast estrogen screen (YES) assays and high performance liquid chromatography (HPLC) analysis respectively. The results show UV alone does not effectively degrade BPA; however, advanced oxidation using UV/ H2O2processes significantly removes BPA parent compound and total estrogenic activity in the solution. Degradation products of BPA are formed during UV/ H2O2 processes, but their identity and estrogenic activity remain unknown. Future research will focus on the fractionation of degradation byproducts from UV-treated water and assess the estrogenic potency for each major byproduct by YES assays and in vivo vitellogenin induction using medaka (Oryzias latipes) fish.
Key words: UV irradiation, bisphenol A, advanced oxidation, yeast estrogen screen