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IP10 Emerging Issues in Analytical Chemistry
(IP084) Matrix-effect free analytical methods for determination of perfluorinated carboxylic acids in environmental and biological samples.
Powley, C1, Ryan, T1, George, S1, 1 DuPont Haskell Laboratories, Newark, DE, USA
ABSTRACT- The sample matrix can often have significant effects upon the performance of analytical methods for the quantitative determination of perfluorinated carboxylic acids in environmental and biological samples. These effects can include inhibition of the extraction procedure due to binding of residues and suppression or enhancement of the signal obtained in LC/MS/MS determinations. We have developed methods for the determination of perfluorinated carboxylic acids in environmental matrices (soil, sediment and sludge) that address these issues, with limits of quantitation of 1 ppb. Extraction efficiency is demonstrated with fortifications prepared using a procedure that mimics field weathering of the residues. Matrix enhancement and suppression are reduced to a negligible extent using isotopically-labeled internal standards when these analogs are available and novel solid-phase extraction (SPE) procedures when they are not. The same procedures are used to purify extracts from biological matrices with similar success, with limit of quantitation of 1-5 ppb. Quality control data have been developed that demonstrates (1) recovery of laboratory fortifications; (2) freedom from matrix enhancement or suppression; (3) recovery of aged residues and (4) absence of significant background interference.
Key words: HPLC/MS/MS, perfluorinated
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