HP4 Environmental Metabolomics
204 Oregon Ballroom
1:20 PM - 5:20 PM, Thursday
() NMR Analysis of Elevated Temperature on Developing Oncorhynchus mykiss Eggs.
Johnson, M1, Turner, M1, Teh, S1, Viant, M2, 1 University of California, Davis, CA, USA2 University of Birmingham, UK
ABSTRACT- The use of Nuclear Magnetic Resonance spectroscopy offers insight into the effects of stressors on metabolic changes during development. This technique provides an important biochemical link between stressors and physiological performance. Eggs from three different steelhead trout Oncorhynchus mykiss females were fertilized by one male and eggs were reared at normal (8°C) and warm (18°C) temperatures to evaluate how temperature stress affects metabolic trajectories during development. We also measured growth, hepatosomatic index (HSI), and skeletal asymmetry, a measure of developmental stability linked to future survival and reproductive success. The eggs were sampled at five different developmental stages and prepared for NMR analysis. Spectral data were binned and reduced using Principal Components Analysis (PCA). Analyses of PC scores demonstrated that developmental trajectories differed between rearing temperatures. There also appears to be genetic differences in developmental trajectories. Mortality for eggs reared at 18°C was significantly greater than for those at 8°C. The fish that were reared in the cooler temperatures also exhibited higher HSI and lower asymmetry. However the condition index of fish at emergence, 30 days post emergence and 60 days post emergence was significantly greater for fish reared at warmer temperatures. It is unknown if these differences are due to changes in metabolite levels, metabolic pathways or a combination of the two. With the development of NMR spectral data libraries we hope to further elucidate what metabolite changes are occurring due to warm incubation temperatures.
Key words: metabolomics, Onhcorhynchus mykiss, temperature, asymmetry