MP7 Advances in Analysis and Measurements
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1:20 PM - 4:40 PM, Monday
() Novel analytical method for glyphosate and its major metabolite in sewage sludge.
GHANEM, A.1, DUBROCA, J.1, KERHOAS, L.1, EINHORN, J.1, 1 INRA, Versailles, FRANCE
ABSTRACT- Disposal of sewage sludge on agricultural lands becomes a major route for its recycling. The environmental risk assessment requires in-depth knowledge of its bio-chemical quality. Data are available in some extent for organic pollutants in the sludge such as the PAHs, PCBs, etc. however, research for pesticides in this matrix is still insufficient. Glyphosate is among the most widely used pesticides. It is a broad-spectrum, foliar-applied herbicide, frequently found in natural waters and suspected in sewage sludge too. Because this pesticide is highly polar and amphoteric, its analysis is very tedious, especially in environmental matrix and needs several steps of sample preparation. The present study describes the development of an analytical method for determination of glyphosate and its major metabolite, aminomethylphosphonic acid (AMPA) in sewage sludge. For the first time, we report herein its extraction from sludge under alkaline or acidic conditions (optimized by using a radio-labeled [14C]-glyphosate), its purification on solid phase supports including an anionic exchanger. An efficient derivatization procedure yielding FMOC-products has been developed using solid phase-adsorbed glyphosate and AMPA. Quantitative analysis was performed with high selectivity and sensitivity by HPLC-MS/MS.
Key words: AMPA, glyphosate, sewage sludge, analytical method