TA5 Soil Ecotoxicology and Risk Assessment
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() New Environment Canada test method using parthenogenetic and sexually reproducing collembolan species.

Becker-van Slooten, K.1, Stephenson, G.2, Miller, J.3, Campiche, S.1, Scroggins, R.4, 1 Laboratory of Environmental Chemistry and Ecotoxicology, Ecole polytechnique fédérale de, Lausanne, Switzerland, Switzerland2 Stantec Consulting, Guelph, Ontario, Canada, Canada3 Miller Environmental Sciences Inc., Innisfil, Ontario, Canada, Canada4 Biological Methods Division, Environmental Technology Centre, Environment Canada, Ottawa, Ontario, Canada, Canada

ABSTRACT- The creation of a new Environment Canada test method for measuring survival and reproduction effects in contaminated soil using springtails is the final phase of a soil toxicity test method development program started in 1994. The goal is to develop a standardized test procedure that is applicable to Canadian soil types using species representative of Canadian soil ecosystems. The collembola considered for the method include the parthenogenetic species Folsomia candida and the sexually reproducing species Onychiurus folsomi. A second sexually reproducing species, Folsomia fimetaria, is currently being assessed by Environment Canada as a possible additional species in the test method. The use of sexually reproducing species was recommended by international experts who participated in an Environment Canada workshop on toxicological assessment of Canadian soils and development of standardized test methods held in February 2003. The test design is a definitive 28- or 35-day test for measuring chronic toxicity using survival, adult fecundity, and number of live progeny as endpoints. This method is harmonized with the ISO standard 11267. A range finding test (7-, 14-day or longer) will also be described in the new method. Key components of the test method and the rationale for selection of test specifications will be discussed. Considerable research has been conducted to support the development of this method. The reproductive success of the species was evaluated in different typical Canadian agricultural and forest soils. Boric acid and 4-nitrophenol were evaluated as potential reference toxicants for use in the test with F. candida and O. folsomi. Boric acid satisfied more criteria for an effective reference toxicant than either 4-nitrophenol or most reference toxicants proposed for soil toxicity assessments. Data to support this choice will be presented and ongoing research with F. fimetaria will be described.

Key words: Folsomia fimetaria, Folsomia candida, Onychiurus folsomi, reference toxicant

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