WA2 Chemical and Biological Analysis of Endocrine Disrupting Compounds
255 Portland Ballroom
8:00 AM - 12:00 PM, Wednesday

() Endocrine Disruption Activity in Fish and Sediments from European Mountain Lakes.

Pina, Benjamin1, Garcia-Reyero, N1, Fernandez, P2, Grimalt, J2, 1 IBMB-CSIC, Barcelona, Spain2 IIQAB-CSIC, Barcelona, Spain

ABSTRACT- The presence of endocrine disruptors was tested in both fish muscle and sediments from lakes Mountain Lakes across Europe using the Recombinant Yeast Assay (RYA). The selected sites covered a geographical area between the Pyrenees in Northern Spain to Central Norway at the North and Bulgaria and Slovakia at the East. In both cases, results revealed a widespread contamination by estrogenic compounds that reached all lake regions. In total, 55 fish muscle samples obtained from 10 lakes, and 83 sediment samples were analyzed both by RYA and by GC-MS. Lakes Velke Hincovo in the Tatra Mountains (Slovakia) and Redo in the Pyrenees (Spain) showed the highest proportion of estrogenic fish muscle samples. Estrogenicity of muscle extracts was strongly correlated with fish age, indicating bioaccumulation of endocrine disruptors. It was also correlated with the concentration of several semi-volatile OCs, like polychlorobiphenyls, g-HCH and HCB. Sediment samples also showed significant levels of estrogenic activity. Comparison of the estrogenicity of the upper half a centimeter of the sediment with samples taken 10 to 15 cm below the surface, corresponding to pre-industrial ages, showed that EDCs were deposited predominantly in recent times. Chemical analysis revealed that high estrogenic sediments were significantly enriched in both Polycyclic Aromatic Hydrocarbons (PAHs) and several Organochlorine Compounds (OCs). Finally, there was a good correlation between estrogenic activities of fish samples and the concentration of PCBs in the sediments of the corresponding lake. These data indicate the usefulness of functional assays for estrogenicity, such as the RYA, to assess the overall human impact in remote areas.

Key words: recombinant yeast assay, endocrine disruption, organochlorine pollutants, ecotoxicology

Internet Services provided by
Allen Press, Inc. | 810 E. 10th St. | Lawrence, Kansas 66044 USA
e-mail assystant-helpdesk@allenpress.com | Web www.allenpress.com
All content is Copyright © 2004 SETAC