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PT13 Aquatic Ecotoxicology I
(PT213) Using Dinoflagellate Bioluminescence as an Indicator of Marine Pollution.
Wolensky, Beth1, Stilts, Corey1, 1 Chatham College, Pittsburgh, PA, USA
ABSTRACT- This study used dinoflagellate (Pyrocystis fusiformis) luminescence as a method to determine the amount of pollutants in marine water samples. Luminescence of dinoflagellates occurs when externally stimulated. A control dinoflagellate colony was established. The lighting of the control group was regulated so that its circadian rhythm was reversed and luminescence could be measured during the day. Control samples were exposed to varying pollutants including oil, PCBs and PAHs. These pollutants were tested at concentrations ranging from 10-3 to 10-6 M. The luminescence of the samples was measured and the polluted samples were retained. Chronic effects of the pollutants were measured by determining luminescence of the retained samples on a daily basis for a period of one week. A correlation of pollutants and exposure length to the amount of luminescence was determined. Physical methods of stimulation (agitation, chemical and light) were also evaluated. The reproducibility and amount of luminescence were assessed for each method of physical stimulation.
Key words: bioluminescence , dinoflagellates
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