PW17 Control and Regulation of Non-idigenous Species
Exhibit Hall
8:00 AM - Wednesday

(PW267) Toxicity of a proposed ballast water biocide, SeaKleen® to embryo and larval stages of Pacific herring.

Dinnel, P1, Marko, L1, 1 Western Washington University, Anacortes, WA, USA

ABSTRACT- Widespread transport of exotic marine organisms in ship ballast water has led to development of a variety of proposed methods to treat ballast water prior to discharge. One chemical treatment proposed for use as a biocide is SeaKleen®, which is a mixture, by weight, of menadione sodium bisulfate and menadione. Experiments to test the efficacy of using SeaKleen as a ballast water biocide have been proposed for the Puget Sound region, with discharge of treated ballast water into Sound waters. However, concerns about SeaKleen toxicity to local fauna, especially to early life stages of the Pacific herring (Clupea pallasi), resulted in bioassay testing of SeaKleen with embryo and early larval stages of herring. Development of Pacific herring embryo and larval testing protocols designed for use in Whole Effluent Toxicity (WET) testing programs has been underway at the Shannon Point Marine Center in Anacortes, WA to provide additional biological tools for testing effluents. Tests of SeaKleen toxicity represent the first application of the new herring test protocols developed at Shannon Point. SeaKleen toxicity test results will be presented for the following test endpoints: 18-day embryo development (normal survival, heart rate, embryo movements, time to 50% hatch, larval length at hatch), 10-day larval survival (LC50), and 10-day larval survival and growth (LC50, larval dry weight).

Key words: toxicity, SeaKleen, Pacific herring, ballast water

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