PARENT SESSION

PM04 Phthalate Esters: Fate and Effects
Exhibit Hall
8:00 AM - Monday

(PM023) Reproductive toxicity by bisphenol A and/or benzyl butyl phthalate during gestation, lactation of rats.

Hwang, SH^{1, 2}, Kang, HJ², Kim, JH², Kim, HS², Kim, KT², Kim, PG², ¹ School of Public Health, Seoul National University, Seoul, Repulic of Korea² Dept. Environmental Health, Yongin Univeristy, Yongin, Kyonggi Do, Repulic of Korea

ABSTRACT- This study has been focused on both developmental and estrogenic activity of bisphenol A (BPA) and/or benzyl butyl phthalate (BBP) to the second generation of Sprague-Dawley rats ingested on gestational or lactational periods. Rats were given BPA 2, 20, 200ug/kg, BBP 5, 10, 100mg/kg of pregnancy or lactation periods. The rats were killed on day 21 of birth. In the F1 female rats treated with BPA on lactational periods, there were significant changes in liver, kidney, uteral weights. In F1 male rats, there were significant changes not only in liver, spleen, kidney but also in testis, epididymis, vesicular glands. The prostate was smaller than the control's. BPA 200ug/kg induced early recovery of estrus in delivered rats. The F1 female rats showed significant organ weight changes in liver, spleen , ovary, uterus exposed on lactational periods. The F1 male rats showed significant organ weight changes in testis, epididymis, vesicular glands, prostate exposed on gestational periods. BBP 5mg/kg or 10mg/kg induced early recovery of estrus in fostering dams. Estrogen receptor expression by BPA and BBP in the uterus (dam, F1 female) and testis (F1 male) were studied. There was no significant different ER expression pattern between control and treated groups. But ER expression were increased in F1 male testis and female uterus. F1 male showed distinct ER expression, especially in the group of lactational combined exposure. Synergistic ER expression was found by combined treatment of BPA and BBP. There were no effects on the body weights changes of dams and neonates treated with low dose of BPA and/or BBP. But, medium and high dose of treatment showed body weight gain retardation. The lactation exposure induced significant changes on relative organ weight. BPA and/or BBP induced early recovery of estrus cycle in fostering dams.

Key words: butyl benzyl phthalate, bisphenol A, second generation, ER