PM15 Biomarkers
Exhibit Hall
8:00 AM - Monday
(PM227) Induction of CYP2M1- and CYP2K1-like proteins in caged Stripey sea perch by produced formation water.
Zhu, S1, CodiKing, S2, Haasch, M1, 1 The University of Mississippi, School of Pharmacy, Environmental Toxicology Research Program, Department of Pharmacology, University, MS, United States2 Australian Institute of Marine Science, Townsville, Qld, Australia
ABSTRACT- Normal operation of oil well platforms results in the discharge of produced formation water (PFW). From 21 to 30 May 2003, a controlled field fish caging study, funded by Apache Energy Pty Ltd, was conducted in order to evaluate the impact of PFW on the expression of biomarkers in fish of the Northwest Shelf of Australia. Previous studies had indicated the possible induction of CYP2 family proteins in Gold-Spotted Trevally (Carangoides fulvoguttatus), and Bar-Cheeked Coral Trout (Plectropomus maculatus; unpublished data). In this study, the expression of CYP2M1- and 2K1-like proteins was examined in the Stripey sea perch (Lutjanus carponotatus) as possible biomarkers of PFW impact. Fish were caught at a clean site, then distributed to three caging sites: A (near field ∼300 m) and B (far field ∼1000 m) and C (a reference site in the Montebello Islands) and sampled at time (T) zero, and T=3 and T=10 days to conduct a controlled field exposure study. Sites A and B were selected to be along a visible surface oil plume. Prevailing winds were calm from T=0 to T=6 then switched between day 6 and day 10 of sampling to 40+ knots, moving the plume due west some of the time. Significant increases in two different immunodetectable proteins for both CYP2K1- and CYP2M1-like proteins were noted at T=3 and T=10 days at Site A (near field) while variable results were noted at Sites B and C. No differences were detected between males and females although there were individual differences in protein pattern of the two proteins of approximately 50 and 54 KD. Taken together these results indicate that CYP2 family proteins are sensitive to petroleum hydrocarbons and may act as good biomarkers of exposure in tropical fish species. (Supported by Apache Energy Party Ltd and the Environmental Toxicology Research Program of UM)
Key words: tropical fish, produced formation water, biomarker, cytochrome P450