PT07 Chemical and Biological Analysis of Endocrine Disrupting Compounds
Exhibit Hall
8:00 AM - Tuesday
(PT117) Evaluation of the estrogenic activity of seawater from the coastal area of Suruga Bay, Japan.
Kurihara, R.1, Ueda, Y.1, Shiraishi, F.2, Tanaka, N.3, Hashimoto, S.1, 1 Institute for Environmental Sciences, University of Shizuoka, Shizuoka, Shizuoka, Japan2 National Institute for Environmental Studies, Tsukuba, Ibaraki, Japan3 Hadano Reseach Institute, Food and Drug Safety Center, Hadano, Kanagawa, Japan
ABSTRACT- We developed an analytical method to measure the levels of some polycyclic aromatic hydrocarbons (PAHs), hydroxy-PAHs (hPAHs), and natural and synthetic estrogenic compounds in seawater. PAHs, such as naphthalene and anthracene (ANT), are ubiquitous environmental contaminants that are present in petroleum products and form as by-products of the incomplete combustion of organic compounds. In this study, we focused on hPAHs, some of which have been reported to have estrogenic activity. For instance, hydroxyanthraquinones (hATQs), which are produced through photochemical reaction of ANT, are known to be toxic. We used the yeast two-hybrid system to evaluate the estrogenic activities of several hPAHs, and carried out estrogen agonist and antagonist tests with or without rat liver S9. Some of the hPAHs showed estrogenic activity: the compound (2-hydroxyanthraquinone) showing the highest estrogenic activity was about one-third less potent than p-nonylphenol. The estrogenic activity of seawater from Suruga Bay, Japan, was evaluated, and the highest estrogenic activities and concentrations of endocrine-disrupting chemicals were observed at a sampling station located near a sewage treatment plant. Seawater from Suruga Bay was also analyzed by gas chromatography/mass spectrometry (GC/MS) to determine the levels of estradiol, estrone, ethynylestradiol, nonylphenol, and bisphenol A in the seawater. The main estrogenic compounds in the seawater were estrone and bisphenol A. However, PAHs such as ANT can be transformed into toxic compounds when they are irradiated by sunlight, so it is important to monitor not only PAHs but also hPAHs in the environment.
Key words: estrogenic activity, hydroxy-PAH, yeast two-hybrid system, Suruga Bay