PM11 Wildlife Ecotoxicology
Exhibit Hall
8:00 AM - Monday
(PM198) Evaluation of impact using AROD activities on small mammals collected in industrial wastes site, Japan.
Yamada, Takao1, Noda, Kaori1, 1 Hirosaki University, Hirosaki, Aomori pref., Japn
ABSTRACT- About 82,000 m3 of hazardous industrial wastes had dumped illegally for more than 10 years on a pastureland at the prefectural border line between Aomori and Iwate, Japan. It was the largest scale of discarded wastes in Japan. Contaminations on adjacent water systems by various toxic substances released from the industrial wastes have been concerned, because the dumping site is located in mountainous area. Although wild inhabitants around polluted area are also thought to be threatened, impacts of hazardous wastes on animals have never been studied. In order to evaluate influences caused by the pollutant has on wildlife around the dumping area, hepatic alkoxyresorufin-O-dealkylase (AROD) activities were determined as biomarker. Heavy metal concentrations in livers and hairs were also analyzed. Three species of small mammals: Greater japanese shrew-mole (Urotrichus talpides), Japanese field vole (Microtus montebelli), and Large japanese field mouse (Apodemus speciosus) were used for analytical samples. A meadow where are separated with a divide, about 2 km south from the dumping spot were chosen as control site. Mercury concentrations in livers of Voles collected in polluted site were significantly higher than control site but those values were not remarkably high. Mercury concentrations at livers and hairs of Shrew-moles were about 30-fold and 120-fold higher than those in Voles, respectively. This might be shown accumulation of heavy metal by biomagnification through food-chain because Shrew-moles are insectivores whereas voles are herbivorous. Field mice caught in polluted area had significantly higher activities of ethoxyresorufin-O-deethylase (EROD) than those from control site. This result suggested that Field mice were influenced by chemical compounds which induced CYP1A1 and CYP1A2.
Key words: AROD activity, small mammals, heavy metal, industrial waste