HA8 On-line Water Toxicity Monitoring
A105 & A106
8:00 AM - 12:00 PM, Thursday
() Development of an on-line biological early warning system using Japanese medaka (Oryzias latipes) for monitoring a mixture of toxic chemicals in water supplies.
Kang, I.J.1, Oshima, Y.2, Kunimaru, H.1, Yamasuga, M.1, Okamoto, F.1, Honjo, T.2, 1 SEIKO Electric Co., Ltd., Fukuoka, Japan2 Kyushu University, Fukuoka, Japan
ABSTRACT- It is increasingly necessary to monitor toxicants in water supplies using aquatic organisms in real-time, because of the possibility that unexpected toxicants and their mixture are released into water supplies. For example, a mixture of several agrochemicals are used in the same season. We developed an on-line fish-sensor system using Japanese medaka (medaka-sensor, SEIKO ELECTRIC CO., LTD, Japan), and we conducted exposure tests using medaka to evaluate the detection ability of the medaka-sensor for the mixtured toxicity of complex chemicals. This system comprised an aquarium (10 × 10 × 15 cm) with a continuous flow system (400 ml/min). Two cameras tracked the behavior of medaka, and images from the cameras were used to calculate the behavior of fish in three dimensions. These data were analyzed on a computer in real-time to detect abnormal or deviant behaviors. In a previous study, we demonstrated that this system could detect, within a short period of time, toxicity of individual chemicals, for example, after 10 min of the exposure at 10 ppm of potassium cyanide (KCN). In the present study, we exposed medaka to a mixture of phenol (12.5 and 50 ppm) and KCN (1 and 10 ppm) for 1 hour, and the medaka-sensor system was able to detect abnormal behaviors. In addition, medaka were exposed to a mixture of several pesticides (fenitrothion, diazinon, benthiocarb and atrazine). Also, the results mixed toxicity test were compared with the results of the individual toxicity test for each chemical. All results of real-time monitoring using the behavior of medaka will be presented.
Key words: on-line monitoring, water quality, medaka, mixture toxicity