PH10 Soil Ecotoxicology and Risk Assessment
Exhibit Hall
8:00 AM - Thursday
(PH135) Screening of Environmental Genotoxicants in Landfilled Soils Samples.
Di Marzio, W1, 2, Sáenz, ME1, Hernández, X1, 1 Programa de Investigación en Ecotoxicología, Departamento de Ciencias Básicas, Universidad Nacional de Luján, LUJAN, BUENOS AIRES, ARGENTINA2 CONSEJO NACIONAL DE INVESTIGACIONES CIENTIFICAS Y TECNICAS CONICET
ABSTRACT- The scope of this work was to perform an environmental screening of potential genotoxicants present in landfilled soils. Eleocytes of Eisenia foetida were exposed in vitro to leachates of each soil. The non-invasive extrusion technique was used for collecting earthworm coelomocytes in a guaiacol glycerol ether free medium. Single cell gel electrophoresis (SCGE) assay was used to primarily measure DNA strand breakage in single cells. This assay was used as a potentially high-throughput screening assay, in mechanistic studies distinguish between genotoxicity versus cytotoxicity induced chromosomal damage; in mechanistic in vivo studies to distinguish between genotoxic versus non-genotoxic carcinogens; and as part of a battery of in vitro/in vivo assays used for regulatory submissions. Data showed a good correlation with cadmium, lead, nickel, mercury, chromium and total hydrocarbons soil concentrations. The genotoxic evaluation, using eleocytes of E. foetida, by SCGE could be a good biomarker of chronic exposure to polluted soils. Since, it would be reflecting the bioavailability, bioaccumulation and biotransformation process.
Key words: heavy metals, landfilled soils, SCGE, genotoxicants