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TA2 Molecular Indicators for Ecological Exposure
() AURATUS: The goldfish environmental genomics project.
Trudeau, V1, Xia, X1, Martyniuk, C1, Jin, X1, Werry, K1, Xiong, H1, Popesku, J1, 1 Centre for Advanced Research in Environmnetal Genomics, University of Ottawa, Ottawa, Ontario, Canada
ABSTRACT- Considerable experimental evidence indicates that most organs and tissues will respond to xenohormones and environmental pollutants. However, there are far fewer environmental and toxicological studies on the neuroendocrine brain than on peripheral tissues. This is surprising given that the brain is the main sensor of environmental change and coordinator of endocrine systems, including those regulating growth and reproduction. With this as a basis we are developing AURATUS, a multifaceted resource for cDNA microarray analysis of gene expression in the goldfish ( Carassius auratus) brain. Within the AURATUS framework are goldSOURCE, an interactive database, goldfEST, a collection of goldfish brain expressed sequence tags and goldMINER, the data mining and management tool. To date we have over 400 verified cDNA sequences derived from a brain-enriched cDNA library and homology cloning of neuropeptides, receptors and enzymes of known importance. These cDNAs are printed on glass microarray slides to analyse the effects of sex hormone mimics and neurotoxins. Progress on AURATUS will be reviewed within the context of molecular indicators of ecological exposures. Supported by NSERC.
Key words: brain, microarry, endocrine disruption, neurotoxicology
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