PM10 Mechanisms of Toxic Action
Exhibit Hall
8:00 AM - Monday
(PM167) Evaluation of deiodinase 1 expression in the offspring of ammonium perchlorate-exposed deer mice.
Gentles, A1, Ramachandran, B1, Smith, E1, 1 Texas Tech University-TIEHH, Lubbock, Texas, USA
ABSTRACT- In this study, deer mice pups were exposed from gestation through lactation to ammonium perchlorate at 3 concentrations: 0ppm, 58.9ppm, and 117.5 ppm. The pups were euthanized at postnatal day 21 and the kidney and liver quickly removed and placed in liquid nitrogen. Total Ribonucleic acid (RNA) isolated from clean animals was used to generate a partial deer mouse specific cDNA sequence for deiodinase 1 (D1) using the method of reverse transcription polymerase chain reaction. This sequence was then used to generate a set of deer mouse specific probe and primers that were utilized in real time PCR to quantitate the relative D1 gene expression in the kidney and liver of treated and control animals. Preliminary results show that AP at 58.9 ppm and 117.5 ppm, reduced the expression of D1 in the kidney to approximately a half that in the kidney of control animals. In the liver, AP also reduced the expression of D1, however, AP at 58.9 ppm reduced D1 expression to one-third the value of the control while at the higher AP dosage D1 expression was two-thirds the control value. These results indicate that perchlorate may affect the thyroid-hormone-axis other than inhibition of iodide uptake.
Key words: perchlorate, deiodinase I, deer mice, kidney liver