MA8 Ecotoxicology of Tropical Aquatic Environments
A105 & A106
8:00 AM - 12:00 PM, Monday

() Biochemical biomarkers in fish caught during MoMAM, a marine monitoring program off the Southeastern coast of Brazil.

Ventura, E.1, Gaelzer, L.2, Fernandes, F.2, Danelon, O.2, Marques, M.1, Bainy, A.1, 1 Lab Biomarcadores de Contaminacao Aquatica e Imunoquimica, Departamento de Bioquimica, CCB, UFSC, Florianopolis, SC, Brazil2 Instituto de Estudos do Mar Almirante Paulo Moreira, Arraial do Cabo, RJ, Brazil

ABSTRACT- The Brazilian coast has highly productive areas, but sensitive and susceptible to contaminant discharges. Contaminants may induce metabolic disorders in the organisms, leading to decreases in the fisheries′ stocks and quality of living resources for human consumption. The International Council for the Exploration of the Sea (ICES) has recommended the use of techniques that may express the degree of exposure and effect caused by contaminants to fish in biological monitoring programs. These include the expression of cytochrome P450 isoform (CYP1A), the activity of glutathione S-transferase (GST) and antioxidant enzymes. The Marine Monitoring Program (MoMAM) was established on December 1996 under the coordination of the Instituto de Estudos do Mar Almirante Paulo Moreira (IEAPM- Brazilian Navy) to evaluate the level of chemicals and the associated effects in fish from the Brazilian coast. Liver samples of Orthopristis ruber and Micropogonias furnieri were collected in different bays of the southeastern coast of Brazil: Arraial do Cabo (AC), Guanabara (GB), Sepetiba (SEP), Ilha Grande (IG) and Sao Sebastiao (SS). In these samples we measured the activity of catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), superoxide dismutase (SOD), glutathione S-transferase (GST) and CYP1A expression. Fish from both species caught at GB showed elevated levels of CYP1A. Likewise, organisms from other sites also showed detectable levels of CYP1A suggesting that most fish were exposed to Ah receptor agonists. Some samples showed indication of hepatic oxidative stress. M. furnieri caught at BG and SS and O. ruber sampled at AC, showed increased xenobiotic conjugating enzyme activities suggesting a more efficient system to conjugate and excrete metabolites. Changes in the expression and/or activity of these biochemical biomarkers in fish caught on the coast of Rio de Janeiro and Sao Paulo, were associated with different degrees of exposure. Supported by IEAPM, UFSC.

Key words: Biological Monitoring, Biomonitoring, Brazil, Biomarker

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