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PT13 Aquatic Ecotoxicology I
(PT193) Use of florescent dye as a toxicity indicator in Ceriodaphnia dubia and Daphnia magna.
Lambert, K.1, Lee, P.1, 1 Department of Biology, Lakehead University, Thunder Bay, Ontario, Canada
ABSTRACT- We are investigating the use of florescent dye added to the food of Ceriodaphnia dubia or Daphnia magna to rapidly indicate the degree of toxicity in pulp and paper TIEs. The theory is that if the organism is stressed it will not eat and this can be determined by the detection of the of florescence in the gut. The advantage is that the method can give an answer much quicker than standard acute or chronic tests. Previous researchers have used the presence or absence of florescent dye in Ceriodaphnia dubia (CerioFAST ™).The problem is that the technique has been an all or nothing response and not particularly appropriate for chronic testing. We have modified this procedure by quantifying the amount of florescence in the gut of Ceriodaphnia dubia and Daphnia magna. The invertebrates are fed using the florescent dye and the amount they eat is quantified using an inverted florescent microscope connected to a computer equipped with an image analysis program. The amount of florescence is then correlated to standard chronic tests to determine their accuracy. In order to make the procedure feasible for industrial applications, we are experimenting with the use of a florescence plate reader. In this procedure, the Ceriodaphnia dubia or Dapnia magna are fed the labeled food, then placed into the small wells present on a plate and the degree of florescence measured.
Key words: Toxicity Indicator Evaluation (TIE), Ceriodaphnia dubia, Daphnia magna, Florescent dye
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