PT07 Chemical and Biological Analysis of Endocrine Disrupting Compounds
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(PT096) Pulp mill effluents effects on trouts in Biobio River, Chile: Use of biochemical and reproductive biomarkers.

Orrego, R1, Moraga, G4, Gonzales , M2, Gavilan , J F4, Valenzuela, A3, Burgos, A2, Barra, R1, 1 Aquatic Systems Research Unit EULA-Chile Environmental Sciences Centre, University of Concepcion., Concepcion, Concepcion, Chile4 Department of Cellular Biology, Faculty of Biological Sciences, University of Concepcion, Concepcion, Concepcion, Chile2 Department of Clinical Biochemistry and Immunology, Faculty of Pharmacy, University of Concepcion, Concepcion, Concepcion, Chile3 Doctoral Program in Biological Sciences, Faculty of Natural and Oceanographic Sciences, University of Concepcion, Concepcion, Concepcion, Chile

ABSTRACT- four pulp mills, which discharge their effluents in the same section of the biobio river south central chile, are the main sources of stress in this area. these discharges produce a very complex mixture whose characteristics depend on the type of raw material, the process technology, and the effluent treatment. to investigate the effect of pulp mill effluent discharges, we used two specific strategies: a laboratory sediment exposure and a caging experiment using fish. three sampling areas were defined in a spatial gradient in the river: pre-impact, impact and post-impact zones of pulp and paper mill discharge areas. in these stations immature trouts (o. mykiss) coming from a cultivation center, were exposed for 30 days, using cages under a density of 4,8 kg/m3. in the laboratory, the same kind of fish were exposed (for 29 d) to sediments obtained from these zones. in both experiments, an induction of erod activity were observed in exposed fish from the impact and post-impact areas, when compared to the pre-impact zone. in addition, histological analysis of the gonad tissues showed a clear induction of fish maturation in the same fish, finding oocytes in a vitellogenic state, while the pre-impact zone only presented immature oocytes. a similar result was observed in plasma, higher levels of vtg in trout exposed to impact and post-impact zones as compared to the pre-impact zone. haematological analysis did not present differences in red cells (erythrocytes), although differences were noted in the white cells (leukocytes + thromobocytes) among the organisms exposed to pre-impact, impact and post-impact sediments in comparison with organisms exposed to the control, which showed some experimental treatment effects. finally, the biomarker approach showed evidence that the sediment associated with pulp mill effluent discharges produces some effects in fish under laboratory conditions and a correspondence was observed in the results obtained in the two experiments. this work was supported by a setac-procter and gamble doctoral research granted to r.orrego

Key words: EROD, Pulp mill effluent, Gonadal maturation, Vtg

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