PT04 Endocrine-Disrupting Chemicals in Wastewater Treatment Effluents
Exhibit Hall
8:00 AM - Tuesday
(PT060) Development of ELISAs for the analysis of EDCs in Wastewater Treatment Effluents.
Fujimoto, S1, Hirobe, M1, Kobayashi, A1, Goda, Y1, Ike, M2, Fujita, M2, Ohkawa, H3, Okayasu, Y4, Shiraishi, H5, Rubio, F6, 1 Japan EnviroChemicals, Osaka, Japan2 Osaka Univ., Suita, Japan3 Fukuyama Univ., Fukuyama, Japan4 Independent Administrative Public Works Research Institute, Tsukuba, Japan5 National Institute for Environmental Studies, Tsukuba, Japan6 Abraxis LLC, Pennsylvania, USA
ABSTRACT- For the analysis of endocrine disruptors and estrogens in wastewater treatment effluents, highly sensitive analytical methods are required because in many instances, contaminants are present at very low concentrations (ng/mL). Instrumental analysis such as HPLC, GC-MS and LC-MS are generally employed for the environmental analysis. These highly reliable analytical methods are, however, have several potential drawbacks including expensive instrumentation, large sample volume, extensive purification, utilization of large volume of solvents, and necessity for technical expertise in their operation. Therefore, there is a strong need for rapid, simple, and cost-effective methods for quantitative analysis of these contaminants. For this purpose, we have developed enzyme-linked immunosorbent assay (ELISA) for estrogens [17beta-estradiol (E2), estrone (E1), total estrogen (ES: E1+E2+estriol (E3)), 17alfa-ethynylestradiol (EE2)], endocrine disrupting chemicals [alkylphenol (AP), AP+alkylphenol ethoxylate (AP+APE), and bisphenol A (BPA)], and surfactants [linear alkylbenzene sulfonates (LAS), alkyl ethoxylates (AE), and (APE)]. In this study, ELISAs for estrogens and alkylphenol, were validated by comparison with instrumental analysis, such as LC/MS/MS or GC/MS(/MS) with environmental water samples including wastewater treatment effluents. Though ELISA tended to overestimate the target compounds in some instances, the values between ELISA and instrumental analysis were well-correlated with optimized sample concentration and clean-up pretreatment. In addition to the test results, sample pretreatment procedures for various ELISAs with wastewater treatment effluents as well as factors causing overestimation for ELISA will be demonstrated in this presentation.
Key words: EDC, ELISA, wastewater treatment effluents, estrogen