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WP2 Chemical and Biological Analysis of Endocrine Disrupting Compounds
() Integrated monitoring for estrogenic compounds using fish (Leuciscus cephalus) as biomonitors.
Grillitsch, B.1, Gemeiner, M.1, Gleiss, M.1, Miller, I.1, Moestl, E.1, Schabuss, M.1, Schober, U.1, Tschulenk, W.1, Walter, I.1, 1 University of Veterinary Medicine, Vienna, Austria
ABSTRACT- Austrian rivers were comprehensively analysed for endocrine disrupters [17beta-estradiol, estriol, estrone 17alpha-ethinylestradiol, 4-nonylphenol, 4-nonylphenol ethoxylates (4-NP1EO, 4-NP2EO) and their degradation products, ocytlphenol, octylphenol ethoxylates (OP1EO, OP2EO), and bisphenol A; chemical monitoring was conducted by the Umweltbundesamt, Vienna, AUT). Chub (Leuciscus cephalus) were examined for estrogenic effects under three scenarios: A) single compound laboratory tests (17alpha-ethinylestradiol; 17beta-estradiol; bisphenol A; Marlophen NP3, 8 % NP1EO, 22 % NP2EO; 4-nonylphenol technical grade); B) by-pass tests with sewage treatment plant effluent (two plants); three weeks duration of exposure in all tests; C) feral fish in three rivers receiving effluents from municipal sewage treatment plants (three sampling locations: above, at and below discharge; three sampling seasons each: spring, summer, autumn). Gonads of fish were analysed for sex of germ cells, maturity, and relative gonadal mass. Plasma levels of vitellogenin were determined (anti-L. cephalus-vitellogenin enzyme immun assay). For one of the rivers studied, fish showed significant differences between sampling sites which were consistent over A) the whole set of indication criteria, B) sampling sites within a river, and C) at least two consequent sampling periods in that male fish from the sampling sites potentially affected by the treatment plant's discharge showed increased plasma vitellogenin levels as well as decreased relative gonadal mass and ripeness compared with males from the sampling site above discharge. Results were confirmed by 'Effect Equivalents' and 'Exposure Equivalents'. For estrogenic compounds in Austrian surface water, risk to fish fauna could not be ruled out. This study was conducted by the 'Austrian Research Co-operation on Endocrine Modulators (ARCEM)' and funded by the Offices of the Austrian Provincial Governments, the Kommunalkredit AG within the framework of the Law on the Promotion of Environmental Measures and the Federal Ministry of Agriculture, Forestry, Environment and Water Management.
Key words: monitoring, endocrine disrupting compounds, biomarker, fish
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